1922] EATON—SULPHUR CONTENT OF SOILS 53 
sulphur pots, but it was not very marked. Also, no definite 
gradation in color from the control series to the high sulphur series 
was discernible. The microchemical analyses were made on 
the clover of the control series and the clover of the high sulphur 
series, in the latter case using mainly plants from series 8, the 
gypsum series. 
The most noticeable point in table X is that the nitrates, pro- 
tein, and sulphates are greater in amount in the plants of the 
high gypsum series. There was not much difference in the carbo- 
hydrate situation in the two series. Leaves from plants of the 
two series had about the same amount of reducing sugars and 
starch. The petioles of the control plants contained more reducing 
TABLE X 
MICHROCHEMICAL ANALYSES OF CLOVER PLANTS OF CONTROL SERIES AND OF HIGH 
SULPHUR SERIES; NITRATES DISCONTINUED May 27 
CaSO, HIGH CaSO, NONE 
Leaves Petioles Roots Leaves Petioles Roots 
Nitrates. .... + ++ ++ + + + 
Protein... ... + ++. + so + + 
Sulphates.. . . ++ a oe ++. isis + + 
IRAE Ss, + ++ a ++ ++ 
eae + +++. ++ + at + 
Sugar than in the case of plants from the high gypsum series, but 
the starch content of the petioles was about the same in both. 
In the roots, the reducing sugar was about the same in amount in 
both series, while the starch was greater in amount in the gypsum 
series. These differences, while clearly evident, were not great 
enough to permit any definite conclusions as to the relation of 
sulphur to chlorophyll development in the clover plant. 
REmer and Tartar (21), Miter (18), DuLey (6), and 
Hart and TorrincHam (9) have shown that root development and 
nodule formation are increased in clover and alfalfa by the use 
of sulphur fertilizers. Prrz (20) has shown that sulphur causes 
an increase in the nodule-forming bacteria of 2-3 fold. REIMER 
and TarTAR have also demonstrated that sulphur increases the 
nitrogen content of alfalfa 2-3 per cent. ScHERTz (26) has shown 
