256 BOTANICAL GAZETTE [NOVEMBER 
and all readings corrected for temperature and barometric pressure, 
the equivalent corrected volumes at o° C. and 760 mm. being 
computed for 1 gm, sample. Ten cc. water and 5 cc. peroxide as 
neutralized were used. 
In the case of the freshly treated seeds, they were soaked for 
ten minutes in the formaldehyde solutions, and kept moist for two 
or more hours, until tested. The moisture of the seeds precluded 
OxYGEn 
cc. 
3 CATALASE 
20 
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TEST 1's 913 260M" a 7H 4ent 17 19 21 22 t@ 20 2224 
Dee eee os 
1G. a sv aeeect of formaldehyde in varying concentrations upon ability of see ed 
extract to ga ei rom m dioxygen: height of lines indicates cubic conti 
— of oxygen computed for 
ex ract from water-soaked seeds (controls) erennye by white, ‘vertical lining indicates 
1-320, diagonal lining form: 7 1-160, 
and solid black formaldehyde 1-80; in all fli iar tested while still mois 
the possibility of passing the material through bolting cloth (23), 
but it was soon found that a material difference in the crop of 
oxygen given off resulted if the seed material was more or less 
glutinous and forming sticky masses, or dry enough to separate 
fairly well on grinding in a mortar with carborundum as an abrasive; 
hence all surface moisture was removed with filter paper before 
grinding up the seeds. Inasmuch as differing moisture contents of 
the differing lots would occur between the first lots tested and the 
last in a long series, the errors incident to this cause were avoided 
