1922] ATWOOD—FORMALDEHY DE 257 
as far as possible by testing consecutively the control seeds and those 
treated with 1-320, 1-160, and 1-80. Suchaseries of four naturally 
fall together for comparison in one group, and many such series 
were made, the combined results of which may easily be seen from 
fig. 11 and table III. With some small variations, the results were 
surprisingly uniform in showing the steady depressing effect of 
TABLE III 
DaTA ON CATALASE TESTS AS SHOWN IN FIG. II 
Concentration | Weight of Volume Tempera- ‘sired volume 
Test no. formalin sample loeet Barometer ture treatment = 
— and test pally 
ee eae Water control] 1.015 | 30.1 748 24 2 25.9 
BS a Water control} 1.005 26.9 748 24 ae 23.4 
ey aes Water control| 1.006 23.5 74 25 7 20.3 
fae ea cna ne control] 1.009 22.4 74 25 8.5 19.3 
a eee I-320 1.008 21.8 74 24 2.5 18.9 
Ge ey 1-320 E.000:4°= 20. 74 24 3-75 17-5 
Pee 1-320 1.004 18.6 74 25 oes 16.1 
Be cys I-320 1.002 | 17.6 74 25 9 15.3 
is ee en eee 1-160 1.006 22.2 74 24 2.96 19.3 
: {oN rae re I-160 I.O15 18.3 74 24 3 15.8 
kp eas I-160 1.007 16-7 74 25 7-5 13.6 
Ee eee Ea I-160 1.002 15.3 74° 25 9 13-3 
ci Ma erin —80 I.O10 14.4 74 24 3 12.5 
1 1-80 1.011 13°60 74 24 3 II.3 
Moe. 1-80 i 10.6 L 25 8 9-20 
Ps Bi Peo a 1-80 .610 1 11.9 74 25 9.5 9.63 
sd eee eae Water control} 1.017 24.6 747 24.5 S 21.1 
13 Water control} 1.012 22.3 745.5 25 7-75 19.1 
i I-320 1.007 19.4 747 24.5 5 16.8 
R00 e. I-320 1.O1o 4 3727 745-5 | 25 8.25 15-2 
y 3 digest eel 1-160 1.003 16.7 747 24.5 §-25 14.5 
Petes epee 1-160 T.01r |} 28-5 945.5 | 45 8.5 13-3 
> fea ah ea —80 1,003 12.7 747 24.5 5-5 11.9 
Ree 1-80 r.0r3 | 12-1 745-5 | 25 8.75 10.4 
As the concentration of the 
formaldehyde on catalase activity. 
formaldehyde rose, the catalase activity as measured by oxygen 
yield fell. 
In the studies of the effects of formaldehyde on catalase activ- 
ity after the seeds had been dried about a month in the labora- 
tory, comparison was made only between the control and the 
seeds treated in 1-80 formaldehyde. Fig. 12 and table IV show 
that there is a definite depressive effect of the treatment on catalase 
activity, but it is by no means so great as soon after treatment. 
