410 BOTANICAL GAZETTE [DECEMBER 
connection seems extremely probable. One other fact should be 
noted. The obviously reproductive structures, found abundantly 
in the cells which lie next to those containing the sporelike bodies, 
are found without these sporelike bodies in the region neighboring 
the meristematic tissue. In other words, the sporelike bodies are 
not found in the cells which abut the meristematic cells. Since the 
structures which looked like germinating spores and appeared to 
contain chromatin also appeared in those very cells where the typical 
sporelike bodies were wanting, the evidence is still stronger for the 
spore interpretation. A few microchemical tests were made to 
determine the exact nature of the cell wall of these bodies, but 
nothing definite was established. 
In L. obscurum the habit of growth of the fungus is markedly 
different. The reproductive bodies are larger, and there are none 
of the small sporelike bodies so persistent in L. lucidulum. All 
the cells of the infected region contain the mycelium, but it is much 
less extensive than in L. lucidulum (figs. 91, 93). Here there are 
two lower cells with a very regular and tight coil of the mycelium, 
which is much denser than shown in the illustration, where clear- 
ness was desired. Above this layer is a region of finer coils, gener- 
ally about one cell in thickness. The coils change their orienta- 
tion slightly in the next layer above, until those of the uppermost 
layer are at right angles to those of the lowest layers. The reason 
for this is probably one of absorption, for the mycelial threads 
finely divide and closely abut the walls of the palisade tissue which 
lies above and which contains reserve starch. These fine threads 
were never observed to enter the palisade cells, nor did their tips 
show swelling. This very evident difference in habit constitutes 
my first reason for believing that the fungus in the two species of 
prothallia under discussion is not the same. The second reason 
involves the reproductive-like structures. A glance at these struc- 
tures at the top and bottom of the plate will reveal organs drawn 
to the same scale, but vastly different in detail. The only possible 
comparison may be found in figs. 97 and 125. The latter may be 
only a later stage of the former. The lefthand cell of fig. 112 
when compared with fig. 97 shows the contrast well. The organs. 
of the last named figure occur only in the lower cells of fig. 93, but 
