--..vij^jijyair', ■• ^-n 



12 Sodium Dodecyl Sulfate-Polyacryl amide Gel Electrophoresis 



(SDS-PAGE, 7.5% Gel) Profile of Anti -lobster PPO 



Antibody 67 



13 The Titer Determination of Antibody against Florida Spiny 



Lobster PPO versus Mushroom, Potato, Florida Spiny 



Lobster, White Shrimp, and Brown Shrimp PPO 69 



14 Analysis of Antigenic Properties of Purified Mushroom, 



Potato, Florida Spiny Lobster, White Shrimp, and Brown 

 Shrimp PPO by Competitive ELISA 72 



15 Profile of Protein Standard (SDS-6H), Purified Mushroom, 



Potato, Florida Spiny Lobster, White Shrimp, and Brown 

 Shrimp PPO, and Crude Lobster PPO Preparation on a 

 Nitrocellulose Membrane 74 



16 Determination of the Specific Reactivity of Anti -lobster 



PPO Antibody with Crude Lobster PPO Preparation, 

 Purified Mushroom, Potato, Florida Spiny Lobster, White 

 Shrimp, and Brown Shrimp PPO as well as SDS-6H Protein 

 Standard as Analyzed by Immunoblotting Technique .... 77 



17 The far UV Circular Dichroic Spectra of Mushroom PPO . . . 79 



18 The far UV Circular Dichroic Spectra of Potato PPO ... . 81 



19 The far UV Circular Dichroic Spectra of Florida Spiny 



Lobster PPO 83 



20 The far UV Circular Dichroic Spectra of White Shrimp PPO . 85 



21 The far UV Circular Dichroic Spectra of Brown Shrimp PPO . 87 



22 Effect of Concentration-related Inhibitory Effect of Kojic 



Acid on Mushroom Tyrosinase (PPO) Activity on DL-^-3,4- 

 dihydroxyphenyl alanine (DL-DOPA) 109 



23 The Concentration-related Inhibitory Effect of Kojic Acid 



on the Oxidation of DL-DOPA and L-Tyrosine by Mushroom 



PPO Ill 



24 The Concentration-related Inhibitory Effect of Kojic Acid ■, 



on the Oxidation of Chlorogenic Acid and Catechol by 



Potato PPO 113 



25 The Concentration-related Inhibitory Effect of Kojic Acid 



on the Oxidation of 4-Methyl catechol and Chlorogenic 



Acid by Apple PPO 116 



ix 



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