LIST OF FIGURES 



FIGURE PAGE 



1 Mechanism of Polyphenol Oxidase-catalyzed Reaction .... 8 



2 Structure of Kojic Acid {5-hydroxy-2-hydroxyniethyl-7- 



pyrone) 14 



3 Effect of pH on the Activity of Polyphenol Oxidase (PPO) 



Isolated from Florida Spiny Lobster and Western 



Australian Lobster 28 



4 Effect of pH on the Stability of Polyphenol Oxidase (PPO) 



Isolated from Florida Spiny Lobster and Western 



Australian Lobster 33 



5 Effect of Temperature on the Activity of Polyphenol Oxidase 



(PPO) Isolated from Florida Spiny Lobster and Western 

 Australian Lobster 35 



6 Effect of Temperature on the Stability of Polyphenol 



Oxidase (PPO) Isolated from Florida Spiny Lobster and 

 Western Australian Lobster 38 



7 Sodium Dodecyl Sulfate-Polyacryl amide Gel Electrophoresis 



(SDS-PAGE, 7.5% Gel) Profile of Polyphenol Oxidase from 



Florida Spiny Lobster (FSL) and Western Australian 



Lobster (WAL) 42 



8 Double Reciprocal Plots for the Oxidation of DL-DOPA and 



Catechol by Florida Spiny Lobster Polyphenol Oxidase 



(PPO) 45 



9 Double Reciprocal Plots for the Oxidation of DL-DOPA and 



Catechol by Western Australian Lobster Polyphenol 



Oxidase (PPO) 47 



10 Sodium Dodecyl Sulfate-Polyacryl amide Gel Electrophoresis 



(SDS-PAGE, 7.5% Gel) Profile of Polyphenol Oxidase from 



Mushroom, Potato, Florida Spiny Lobster, White Shrimp, 



and Brown Shrimp 62 



11 Profile of Anti -lobster PPO Antibody Production in 



Immunized Hen during the Immunization Period 65 



viii 



