Table 11 



185 



Secondary Structure Estimates of Nontreated Control and High 

 Pressure CO -treated Florida Spiny Lobster, Brown Shrimp, and 

 Potato Polyphenol Oxidases (PPOs) from Far UV Circular Dichroic 

 Spectra 









% of secondary struct 



ure 



PPO 



a-helix 



24.4 

 19.7 



/3-sheet 



^-turn 



random coil 



Lobster 



Control 

 CO^-treated 



26.2 

 25.9 



21.4 

 15.2 



29.9 

 39.3 



Brown 

 shrimp 



Control 

 C02-treated 



20.1 

 29.6 



22.3 

 18.9 



15.2 

 18.2 



42.4 

 33.3 



Potato 



Control 

 CO^-treated 



14.8 

 17.8 



34.6 

 35.9 



28.4 

 25.9 



21.2 

 20.4 



The circular dichroic spectra of PPO was scanned at the far U.V (250 - 

 200 nm) range. Four-mL PPO (10 /zg/mL) in 0.05 mM sodium phosphate buffer 

 (pH 6.5} was analyzed at ambient temperature. 



