TROPICAL INFANTILE DYSENTERY. 

 Table II. — Bacillus "S." 



35 



Media. 



24 hours. 



48 hours. 



72 hours. 





+ 

 + 

 + 



+ 

 + 

 + 



+ 

 + 











The fermentation tubes inoculated with B. coli showed more gas in 

 the lactose medium than in any of the others. 



PATHOGENICITY. 



Transfers were made to slant alkaline-agar tubes and placed in the 

 incubator at 37° C. for twenty-four hours. The slants were completely 

 covered with the growth and each was then in each instance suspended 

 in 20 cubic centimeters of normal salt solution. One cubic centimeter 

 of this suspension (0.05 of an agar slant) injected intraperitoneally, 

 hilled a guinea pig in twelve hours, 0.04 of a slant was fatal in less 

 than twenty-four hours. Rabbits were much less susceptible, 0.1 to 0.07 

 of a 24-hour slant, inoculated intraperitoneally, killing a rabbit in from 

 twenty-four to thirty-two hours. Similar conditions were found in both 

 rabbits and guinea pigs at autopsy, there was injection of the tissues 

 about the point of inoculation, enlargement of lymphatic glands, a large 

 amount of sero-purulent exudate, and both the liver and spleen were 

 congested; stained smears from the exudate and from the liver and 

 spleen showed the presence in pure culture of a small bacillus, identical 

 morphologically with the original organism. Hanging drops of the 

 exudate demonstrated an organism having a marked motility and in 

 some of the specimens examined, an almost cholera-like character. 

 Subcultures made from the exudate developed in a manner identical with 

 that of the original organism. A rabbit inoculated subcutaneously with 

 peritoneal fluid from a guinea pig dead of the infection developed a 

 large, fluctuating abscess and at the same time showed a very marked 

 diarrhoea. 



Suspensions of the bacillus in salt solution were fed to a monkey 

 through a stomach tube for four succesive days, after the gastric acidity 

 had been neutralized with sodium carbonate. The monkey became ill, 

 at the end of two weeks the animal was killed and autopsy performed. 

 There appeared to be a certain amount of inflammation of the large 

 intestine and caecum and of a portion of the small intestine adjoining. 

 Sections were made and examined microscopically, but they showed 

 nothing which would account for the reserve prostration. Cultures from 



