TROPICAL INFANTILE DYSENTERY. 

 Table III.— Serum from Case I. 



37 



Organism. 



Aggluti- 

 nation. 





+ 















One rabbit (number 3227) was rendered immune to the original 

 Bacillus "8" and another (number 3229) to Bacillus "S" isolated from 

 Case II. Living cultures were used and inoculated intravenously in 

 increasing doses. Tests were made constantly and the agglutinative 

 limit was reached in six weeks, each rabbit was then bled and aggluti- 

 nation reactions made as shown in Tables IV and V. 



Table IV. — Serum from rabbit immunized to Bacillus "S" (Case I) incubated at 

 37° C. for three hours. 



Bacillus. 



1-50. 



1-100. 



1-200. 



1-400. 



1-800. 



1-1,600. 



1-3,200. 



1-6,400. 



1-12,800. 



1-25,600. 





+ 



+ 

 + ? 



'+ + 



+ 

 + 



+ + 



+ + 



+ 



+ 

 + + 

 + + 



+ + 



+ 



+ 



+ + 



+ 



+ 

 + + 

 + + 



+ 

 + + 



+ 



+ 

 + 

 + 



+ ? 

 + 

 + ? 



" 



"L" 



"T"_ _ 





Dysenteric 



Typhi.:. 



Table V. — Serum of rabbit immunized, to Bacillus "S" (Case II) incubated at 37° C. 

 for three hours. 



Bacillus. 



1-50. 



1-100. 



1-200. 



1-400. 



1-800. 



1-1,600. 



1-3,200. 



1-6,400. 



1-12,800. 



1-25,600. 



"L" 



+ + 

 + 



+ + 

 + 

 + 



+ + 



+ 

 + + 



+ 



+ + 

 + 



+ + 



+ + 



+ + 

 + + 



+ 



+ + 

 + 

 + 



+ 

 + 

 + 



+ 

 + 



+ 

 + 



+ 



+ 





"J" 





Dysenteric 



Typhi 









All the tests were made microscopically, test tubes containing suspen- 

 sions of the organism in normal salt solution being used. After each 

 dilution was made, all were placed in the incubator at 37° C. for three 

 hours before being examined. -Controls were made in each case. The 

 seemingty absolute specificity of the serum from each rabbit for the three 

 organisms isolated is very striking, Shiga, colon, and typhoid bacilli being 



