168 RTJEDIGER. 



No. 2. — Virulent blood was diluted with four times its volume of phys- 

 iological salt solution and the mixture divided into four parts designated 

 as a, b, c, and d. 



Part a was not filtered, b was passed through a Berkefeld filter V, c 

 was filtered through a Berkefeld candle marked N, and d was passed 

 though one marked W. 



Four bovines, Eos. 5, 6, 7, and 8 were inoculated with these materials. 



Animal No. 5 received 50 cubic centimeters of a, it developed cattle plague on 

 the fourth day after inoculation and was bled to death on the tenth. (See 

 Chart No. 5.) 



Animal No. 6 was inoculated with 50 cubic centimeters of 6 and remained 

 well. (See Chart No. G.) Twenty-eight days later the animal received 10 

 cubic centimeters of virulent blood, it showed symptoms of cattle plague on the 

 third day and was bled to death on the sixth. (See Chart No. C.) 



Animal No. ?' was injected subeutaneously with 50 cubic centimeters of c. 

 No symptoms appeared. (See Chart No. 7.) Seventeen days later it was inocu- 

 lated with 10 cubic centimeters of virulent blood, it developed cattle plague and 

 was bled to death on the eighth day after inoculation. (See Chart No. 7'.) 



Animal No. 8 received 50 cubic centimeters of d, it remained well. (See 

 Chart No. 8.) After one month 10 cubic centimeters of virulent blood were 

 injected, the temperature began to rise within twenty-four hours and the animal 

 was found dead on the morning of the sixth day. (See Chart No. 8'.) 



No. 3.— One part of virulent blood was laked in four parts of distilled 

 water and the resulting solution was divided into four parts, a, b, c, 

 and d. 



Part a remained unfiltered, b was passed through a Berkefeld filter 

 V, c was filtered through a Berkefeld candle N, and d was passed through 

 a Berkefeld filter marked "W. 



Of four bovines, Nos. 9, 10, 11, and 12, animal No. 9 was inoculated 

 with 50 cubic centimeters of a. The animal showed cattle plague on the 

 third day after inoculation and was bled to death on the seventh. (See 

 Chart No. 9.) 



Animal No. 10 received 50 cubic centimeters of 6 under the skin. It remained 

 well. (See Chart No. 10.) On the nineteenth day 10 cubic centimeters of 

 virulent blood were injected, this procedure was followed by cattle plague in 

 four days. (See Chart No. 10'.) 



Animal No. 11 was inoculated with 50 cubic centimeters of c. This proved 

 to be noninfectious. (See Chart No. 11.) Eighteen days later the same animal 

 was inoculated with 10 cubic centimeters of virulent blood, it sickened with 

 cattle plague in two days and was bled to death on the fifth day after inocula- 

 tion. (See Chart No. 11'.) 



Animal No. 1% received 50 cubic centimeters of d and remained well. (See 

 chart No. 12.) After twenty days it was injected with 10 cubic centimeters of 

 virulent blood. Cattle plague manifested itself on the fifth day and the animal 

 was bled to death on the eighth. (See Chart No. 12'.) 



No. If. — This experiment is an attempt at repeating the work of 

 Nicolle and Adil-Bey and that of Yersin. 



