FURTHER FILTRATION EXPERIMENTS WITH VIRUS OF 

 CATTLE PLAGUE. 



By E. H. "Ruedigek. 



(From the Serum Section of the Biological Laboratory, Bureau of Science, 



Manila, P. I.) 



It was shown in a previous report 1 that the laked or unlaked blood and 

 the bile of bullocks sick with cattle plague, lost the power of transmitting 

 the disease to other animals after being passed through Berkefelcl filters 

 marked V, N or W; while peritoneal fluid retained this property on 

 being passed through either one of these filters. 2 



It was considered necessary to repeat the experiments with peritoneal 

 fluid with all possible precautions, owing to the danger of accidental 

 infection of the animals used for the previous experiment. The bullocks 

 used in the former experiments were kept in a fly-proof stable and so 

 far as could be determined, there was no cattle plague within a radius 

 of three miles, neither did the attendants, feed, utensils, etc., come in 

 contact with animals suffering from that disease, nor with excreta from 

 such animals. 



The results here reported were obtained with peritoneal fluid after it 

 had been passed through one of the following filters : Berkefeld N, 

 Berkefeld W, Chamberland F, and Chamberland B. Five liters of 

 citrate solution (the addition of 0.5 per cent solution of potassium or 

 sodium citrate became necessary to prevent subsequent clotting of the 

 fluid) was injected into the peritoneal cavity of a bullock sick with cattle 

 plague. About one hour later the animal was bled to death, the abdomen 

 opened and the peritoneal fluid collected. 



EXPERIMENTAL. 



No. 1. Peritoneal fluid obtained as above on the 13th day of January was 

 divided into two parts, a and 6. Fifty cubic centimeters of part a, unfiltered, 

 were injected under the skin of bullock No. 27. The temperature of the bullock 

 rose rapidly three days later, and on the 18th day of January, five days after 

 inoculation, the animal was bled to death and the lesions found post-mortem 

 were those characteristic of cattle plague. (See Chart No. 27.) 



1 This Journal (1908), 3, 1G5. 



2 The Berkefeld filters V, N, and W are coarse, medium, and fine grained, 

 respectively. The filter marked W, the finest grained Berkefeld filter, is consider- 

 ably coarser than the Chamberland filter marked F. 



319 



