820 RUEDIGER. 



Part b was passed through a Berkefeld filter marked N and 50 cubic centi- 

 meters of it injected under the skin of bullock No. 28. Cattle plague set in 

 on the fourth day and the animal was bled to death on the sixth day after 

 inoculation. (See Chart No. 28.) 



Bullock No. 48, kept as a control, was not inoculated; it remained well. 

 (See Chart No. 48.) Fifty cubic centimeters of unfiltered fluid were injected 

 on the 11th day of March. The animal sickened with cattle plague and was 

 bled to death on the fifth day after inoculation. 3 



No. 2. Peritoneal fluid received on the 11th day of March was divided into 

 four parts: a, b, c, and d. Part a remained unfiltered; part b was passed through 

 a Berkefeld filter marked W; part c was passed through a Chamberland filter 

 marked F ; and part d was passed through a Chamberland filter marked B. 



Bullock No. 48 received subcutaneously 50 cubic centimeters of part a. It 

 sickened with cattle plague. (See Chart No. 48.) 



Fifty cubic centimeters of part b were injected under the skin of Bullock 

 No. 68 which sickened with cattle plague and was bled to death on the fifth 

 day after inoculation. (See Chart No. 68.) 



Bullock No. 69 was inoculated with 50 cubic centimeters of part c, and showed 

 an irregular temperature for some time following the inoculation, but there was 

 no manifestation of cattle plague. (See Chart No. 60.) On the 27th day of 

 April, 50 cubic centimeters of unfiltered peritoneal fluid were injected under 

 the skin of this animal which sickened and was bled to death on the 3d day 

 of May, six days after inoculation. The diagnosis of cattle plague was verified 

 post-mortem. (See Chart No. 69.) 



Attention was called in my previous report to the fact that Xicolle 

 and Adil-Bey and Yersin found that the etiologic factor of cattle plague 

 may at times pass through the pores of the Chamberland filter marked F, 

 probably due to difference in the size of the pores in different filters. 

 Considerable variation is found in the stream of water from a number 

 of filters, supposed to be of the same grade, such as Chamberland F for 

 instance, delivered by them under identical conditions as shown by the 

 following test : 



Five new Chamberland filters were tested with distilled water. The first 

 delivered a liter of filtrate in five minutes, the second one in eight minutes, the 

 third in eighteen minutes, the fourth in eleven minutes, and the fifth in sixteen 

 minutes. Hence, one can easily understand how different results may be 

 obtained with filters of the same grade. As only one Chamberland F filter 

 was used in this experiment on cattle plague, the result must not be considered 

 conclusive. 



Fifty cubic centimeters of part d were injected under the skin of bullock 

 No. 70. It remained well. (See Chart No. 70.) On the 27th day of April 

 this animal was inoculated with 50 cubic centimeters of unfiltered peritoneal 

 fluid. Cattle plague followed. (See Chart No. 70.) 



Bullock No. 71. kept as a control, was not inoculated and remained well. 

 (See Chart No. 71.) The immunity test was applied on the 27th day of 

 April and cattle plague followed the injection of 50 cubic centimeters of un- 

 filtered peritoneal fluid. (See Chart No. 71.) 



3 Charts Nos. 27, 28, and 48 show a decided rise of temperature on the 9th 

 day of January. On this date the animals were driven a distance of three 

 miles to the laboratory of the Bureau of Science, but it was a very warm 

 day and the animals, practically wild, became overheated. 



