A STUDY OF FIXATION REACTIONS. 



363 



without extracts, for fresh serum in 0.2 cubic centimeter quantities never deflects 

 alone, but on the contrary, increases the rapidity of lysis because of the ambocep- 

 tors for sheep's corpuscles contained in normal and luetic human serum. The only 

 exception to this rule is that the serum from an animal which has just had a 

 hearty meal may cause deflection and under any circumstance this serum is not 

 useful because of its milkiness. All authorities agree upon the necessity of 

 having these various controls. 



d. Tables. — The following tables from various authorities illustrate the manner 

 of setting the deflection test for the determination of the antibody content of the 

 suspected serum. 



Wassermann, Neisser, Bruck and Schucht(2) give the following summary of 

 one of their experiments : 



Immune serum: 



Obtained from a monkey inoculated and subsequently immunized with 

 syphilitic material. 

 Antigen: 



1. Extract of organs from an infant with congenital syphilis. 



2. Extract of bone marrow of a monkey killed seven weeks after inoculation 



with syphilis. » 



Antigen controls: 



1. Extract of organs from a non-syphilitic foetus. 



2. Extract of bone marrow of a monkey free from syphilis. 



Table I. 



.1 



0.1 immune 



0.1 extract lue- 



Fresh gui- 



Union for 



0.002 rabbit 



1 c. c. of 



Block- 





serum. 



tic foetus. 



nea pig 



1 hour 



serum hse- 



5 per cent 



ing of 









comple- 



at 37°. 



m o 1 y t i c 



suspen- 



ha3 mo- 









ment 0.1. 





for sheep 

 corpuscles 

 (2 dissolv- 

 ing doses). 



sion of 

 sheep's 

 blood. 



lysis. 



2 



do 



0.1 extract lue- 

 tic monkey. 



do .... 



do— _ 



do 



do — 



Do. 



3 



do 



0.1 extract nor- 

 mal fcetus. 



___:_do .... 



do.... 



do 



do—. 



Complete 

 haemo- 

 lysis. 



4 



do 



0.1 extract nor- 

 mal monkey. 



do.._. 



do.... 



do 



do.— 



Do. 



5 



0.1 normal 

 monkey 

 serum. 



0.1 extract lue- 

 tic foetus. 



do—. 



do — 



do 



do._._ 



Do. 



6 



do 



0.1 extract lue- 

 tic monkey. 



do .... 



do.... 



do 



do .... 



Do. 



- 



0.1 immune 



Salt solution 



do 



do 



.... do .... 



do 



Do. 





serum. 















8 



0.1 normal 

 monkey 



do . _ .... 



do . 



. .do . _ 



do 



do — 



Do. 











9 



Salt solution 



0.1 extract lue- 

 tic fcetus. 



do — . 



do .... 



do 



do .... 



Do. 



10 



do 



0.1 extract lue- 



do.— 



do 



do 



do_— 



Do. 







tic monkey. 













11 



do 



0.1 extract nor- 

 mal fcetus. 



do — 



do.... 



do 



do — 



Do. 



12 



do 



0.1 extract nor- 

 mal monkey. 



do .... 



do — 



do 



do.... 



Do. 



