364 



MARSHALL AND TEAGUE. 



The other controls which are not tabulated are : ( 1 ) the haemolytic system 

 alone; (2) blood corpuscles in salt solution; (3) blood corpuscles with comple- 

 ment alone; (4) blood corpuscles with haemolytic amboceptor alone; (5) organ 

 extract with blood corpuscles. 



The volume of fluid in each tube is brought up to 5 cubic centimeters with salt 

 solution. 



In this table, numbers 1 and 2 are the only tubes showing the real experiment, 

 all of the others, including the five noted below the table, being employed as 

 controls. These two tubes demonstrate that there is specific luetic material in 

 the organ extracts made from the syphilitic fcetus and the syphilitic monkey, and 

 that there are specific luetic antibodies in the serum of the immunized monkey. 



Wassermann, Neisser, Bruck and Schucht(2) also use a simple form of table 

 indicating only the combinations which are brought together in the presence of 

 complement serum, together with the final result upon haemolysis. 



Table II. 



[Wassermann, Neisser, Bruck and Schucht ; Table I modified. 1 ] 



Serum of 

 normal 

 monkey. 



Serum of 



luetic 

 monkey. 



Extract 

 of non- 



luetic 

 child's 



liver. 



Extract 



of luetic 



child's 



liver. 



Result. 





0.1 





0.1 

 0.1 



Blocking of haemolysis. 

 Complete solution. 



Do. 



Do. 



Do. 



0.1 





0.1 









0.1 





0.1 



0.1 







Meier (3) uses the following table to indicate whether a suspected serum "A" 

 contains antibodies, which will produce deflection with a syphilitic extract and 

 with no other. In this table the actual experiment is shown in column I, numbers 

 1 and 2, while the other tests are controls. In the last column is recorded the 

 degree of deflection. 



He does not tabulate the haemolytic system with its controls. 



Table III. 



Serum. 



I 



I. 



II. 



III. 



End result. 



3 



Result. 





Result. 



3 



Result. 





0.2 



0.1 

 0.2 



0.2 



0.2 



0.1 

 0.2 



0.2 



0.4 



Complete 

 blocking. 



do 



do 



Complete 

 haemoly- 

 sis. 



0.2 



0.2 

 0.2 



0.2 



Complete 

 solution. 



do 



do 



do 



1.0 



1.0 

 1.0 



1.0 



1.0 

 1.0 



Complete 

 solution. 



do 



do 



do 



do 



do 



Serum A = 

 + + + + 

 isasyph- 

 ilitic se- 

 rum. 



Do 



Standard syph- 

 ilitic serum. 

 Normal serum. 

























A = Serum to be tested. 



1 Ztschr. f. Hyg. u. Infectionskranhli., Leipz. (1906), 55, 455. 





