A STUDY OF FIXATION REACTIONS. 



367 



ing 1 hour at room temperature the mixtures are added to the haemolytic com- 

 plex= (0.0015 antiox-rabbit+ 1 cubic centimeter 5 per cent sheep's red blood 

 corpuscle suspension). 



Table IV. 



Amount 



of 

 serum. 



Hemolysis produced with serum from— 







Man. 



Monkey. 



Rat. 



Pig. 



Goat. 



Rabbit. 



Ox. 



Horse. 



.01— 











Complete 



Complete 



Complete 



Complete 



Complete 



Complete 



. 001— 











— -do— . 



t— .do— - 



.... do 



.... do— . 



.... do— . 



Do. 



. 0001.. 







Moderate 



._do_.. 



do .... 



—do .... 



do 



—do — 



Do. 



. 00001_ 



Trace 



Complete 



... _do — 



—.do — 



do 



do... . 



— do— - 



Do. 



. 000001 



Complete; 



—*.do .... 



do 



— -do— . 



—do — 



do— . 



do 



Do. 



0.0 



— -do— . 



.... do.— 



do 



do 



do 



— do— _ 



....do.... 



- Do. 



In this table the presence or absence of haemolysis is noted. It must be 

 remembered that deflection is indicated by the absence of haemolysis, so that the 

 first three or four tubes with human serum, and the first two with monkey serum 

 are the only ones in which deflection has taken place. 



The table of Schiitze, No. V(14) shows deflection with human serum. The 

 sheep's corpuscles and hemolytic serum are mixed separately and not added until 

 the other materials have been mixed and incubated for one hour at 37°. 



Table V. 

 [Each ingredient = 1.0 cubic centimeter, total volume 5 cubic centimeters.] 



Human 

 serum. 



Inactive 



anti- 

 human 

 serum. 



Guinea- 

 pig serum 

 comple- 

 ment. 



Antisheep 

 serum. 



Sheep cor- 

 puscles, 5 

 per cent. 



Result. 



.01 



0.1 



0.1 



.002 



5 



Complete blocking of haemolysis. 



.001 



0.1 



0.1 



.002 



5 



Do. 



.0001 



0.1 



0.1 



.002 



5 



Do. 



. 00001 



0.1 



0.1 



.002 



5 



Appreciable blocking of haemolysis. 



. 000001 



0.1 



0.1 



.002 



5 



Haemolysis. 













Complete hEemolysis. 



salt sol. 











0.0 



0.1+1 cc. 

 salt sol. 



0.1 



.002 



5 



Do. 



0.0 







0.1+2 cc. 



salt sol. 



.002 



5 



Do. 



0.0 











.002+3 CC. 

 salt sol. 



5 



No haemolysis. 



0.0 











o 



5+4 cc. 

 salt sol. 



Do. 



