TUBERGULO-TOXOIDIN AND IMMUNIZATION SEBUM. 381 



(2) Prophylactic test. — Guinea pigs injected subcutaneously with 1.0 cubic 

 centimeter of the toxoidin are generally found to be immune against the inocula- 

 tion of tubercle bacilli from the fourth until the fourteenth day after the 

 operation. 



(3) Therapeutic experiments. — If the treatment of a guinea pig subcutaneously 

 inoculated with tubercle bacilli commences within one week of the inoculation, 

 and 0.5 to 1.0 cubic centimeter of the toxoidin is injected subcutaneously about 

 ten times, the disease will either be cured or prevented from making further 

 progress. If, two or three weeks after the inoculation of bacilli, the injection 

 of the above doses is made into an animal with greatly swollen glands, the swelling 

 subsides, the body weight increases, and the fatal period is postponed; whereas 

 a control animal dies in three months, the test animal receiving injection treat- 

 ment lives over a year. When such an animal is killed, autopsy demonstrates 

 that the tuberculous lesions of the organs are not entirely healed. This is due 

 to the fact that guinea pigs are too susceptible to tubercle bacilli to allow of a 

 complete cure. 



A noteworthy fact is that in the guinea pigs treated with the toxoidin, 

 the visceral tubercles generally show a tendency to heal and the number 

 of cells containing bacilli is much greater than in those which have not 

 been thus treated. Moreover, the bacilli in the cells are small and short, 

 evidently representing a degenerate form. 



CLINICAL APPLICATION. 



From my own experience and the reports of other practitioners who 

 have tried the preparation, the following conclusions may be drawn: 



( 1 ) By injecting the preparation in a gradually increasing dose into tuber- 

 culous patients without fever, almost every one of them increases in body weight 

 and vital capacity, and becomes conscious of the alleviation of the symptoms. 



(2) The bacilli in the sputum are gradually broken up and agglutinated and 

 finally disappear, although in some rare cases small amount of expectoration 

 containing bacilli are found for a long time. 



(3) The quantity of opsonin in the patients' blood is found gradually to 

 increase by the injection treatment. 



(4) The incipient and feverless tuberculous patients, almost without excep- 

 tion, can be completely cured from within three to six months by injection of 

 this preparation. 



(5) In patients in a more or less advanced stage, if nutrition is good, similar 

 results can be obtained. In feverish patients, a satisfactory result is often 

 obtained by means of the injection used side by side with antipyretics. In more 

 serious cases, beyond a certain degree, the treatment is quite useless. 



(6) Those patients who are once cured or alleviated by this treatment only 

 very seldom suffer from a second attack. 



(7) Out of a total of 772 tuberculous patients, each of whom had received 

 more than fifteen injections of tuberculo-toxoidin in my clinic within the past 

 few years, there were 274 who were completely cured and 258 who were partially 

 cured. These last two figures added together make 532, being 68.91 per cent 

 of the total number of patients. Those who for various reasons discontinued 

 the treatment numbered 107; those who died numbered 29; the remainder, 104. 



(8) Out of a total of 778 patients treated with the tuberculo-toxoidin (injected 



