408 ' RUEDIGER. 



Ten bullocks, nonimmune to cattle plague, were now divided into 

 two series, X and Y, with five bullocks in each series. The potency of 

 serum X was determined on the bullocks in series X, and the potency of 

 serum Y on the bullocks in series Y as follows : 



series x. 



Five bullocks, numbered 78, 79, 80, SI and 82, received 0.5 cubic centimeter of 

 virulent blood injected under the skin and varying quantities of serum X. 



Bullock No. 7S, used as control, received no serum and died of cattle plague 

 on the tenth day after inoculation. (Chart No. 78.) 



Bullock No. 79 received 15 cubic centimeters of serum X. It went through 

 an attack of cattle plague of moderate severity and recovered. (Chart No. 79.) 



Bullock No. 80 was given a subcutaneous injection of 30 cubic centimeters of 

 serum X. On the fifth day after inoculation reaction manifested itself by a rise 

 of temperature. On the eleventh day the temperature dropped to normal and 

 the animal made an uneventful recovery. ( Chart N°- 80. ) 



Forty-five cubic centimeters of serum X was injected into bullock No. 81. 

 Five days later the temperature rose to 40°.5 C, but soon dropped to normal 

 and the animal recovered without having shown any clinical signs of cattle 

 plague. (Chart No. 81.) 



Bullock No. 82, having received a subcutaneous injection of 60 cubic cen- 

 timeters of serum X, showed a more marked reaction than did bullock No. 81 

 with the smaller dose of serum. Clinically he had loss of appetite for a few 

 . days. Diarrha?a never set in and recovery was rapid. (Chart No. 82.) 



Bullocks numbered 83, 84', 85, 86 and 97, were each inoculated with 0.5 cubic 

 centimeter of virulent blood and received varying quantities of serum Y. 



Control bullock No. 83, which did not receive any serum died on the eighth 

 day after inoculation. (Chart No. 83.) 



Bullock No. 84, having received 15 cubic centimeters of serum Y, developed 

 typical cattle plague and died ■ on the tenth day after inoculation. (Chart 

 No. -84. ) 



Thirty cubic centimeters of serum Y were injected under the skin of bullock 

 No. 85 ; this animal died of cattle plague on the fourteenth day after inoculation. 

 (Chart No. 85.) 



Bullock No. 86 received an injection of 45 cubic centimeters of serum Y. 

 After passing through a reaction of moderate severity, this animal made an 

 uneventful recovery. (Chart No. 86.) 



Bullock No. 87, having received 60 cubic centimeters of serum Y, had rather 

 a severe reaction. Diarrhoea set in on the eleventh day after inoculation and 

 continued throughout the twelfth day. On the twelfth day the temperature 

 dropped to normal and remained normal ; the diarrhoea suddenly subsided and 

 rapid recovery followed. (Chart No. 87.) 



CONCLUSIONS. 



1. On comparing the results obtained with serum X with those obtained 

 with serum Y, it was found that serum X had by far the higher potency. 



2. The quantity of virulent material used for inoculating serum 



