STREPTOTHRICOSIS. 451 



much as jsossible any influence of long cultural environment were, where 

 feasible, first passed through animals and the studies then made from 

 the reclaimed cultures taken from the experimental lesions. All the 

 cultural and other biologic properties have so far as we have been able 

 to do so, been studied under the same conditions in regard to media, 

 tem]3erature, and generation from animal lesions. Our results and 

 consequent descriptions show some variation from previous descriptions 

 of some of these organisms, but in general the picture is very much as 

 given by the original authors. 



"We have failed entirely to obtain a culture of the Wolf and Israel 

 and Wright type of Actinomyces, but, because of the great importance 

 of this organism in the discussion, we have, for comparative purposes, 

 taken James Homer Wright's description of it from his classical mono- 

 graph. We have also failed to secure a definite culture of Bostroem's 

 Actinomyces , although the one sent by Professor Binot from the Pasteur 

 Institute is probably of this species. However, in view of a possible 

 mistake we have used both the description given by Bostroem as well 

 as the Pasteur Institute culture in our comparative work. The impor- 

 tant characteristics of our cultures are shown in the following descriptions 

 and are summarized in the attached table. 



No. 1. Streptothrix freeri. This organism was isolated by us from a ease of 

 the ochroid variety of mycetoma and has already been fully described (This 

 Journal, Sec. B (1907), 2, 477). It is identical with Eppinger's organism which 

 will be discussed subsequently in this paper, and the name given by us in the 

 first publication should fall as a synonym for S. eppingeri, the latter having 

 priority. Other described organisms of the group which are probably identical 

 with this are those of Stokes, Aoyama and Miyamoto, MaeCallum, Sabrezes and 

 Riviere, and possibly several others. 



No. 2. Streptothrix madurw. This organism has been carefully described by 

 Vincent, Foulerton, and others. A summary of its characteristics are given both 

 in the text and table and in our first report and are again shown in the table 

 attached to this paper. Our work with cultures of this species has been in the 

 main confirmatory of the published results of others. We have worked with 

 three cultures received respectively from Foulerton, Binot, and Strong. These 

 strains are identical in every particular except that the one from Binot is less 

 pathogenic for monkeys than either of the other two. 



Morphologically this species gives very constant results. The colonies are 

 made up of intertwining, long filaments which may, or may not, be radially 

 placed at the periphery. There is true branching, but no club formation in 

 cultures and none in experimental lesions in monkeys up to fourteen days. The 

 organism takes the usual aniline stains; it is Gram positive, but is neither acid 

 nor alcohol fast. 



Culturally it is a facultative aerobe with strong oxygen requirements. It 

 grows well in the incubator and at tropical room temperature. Positive trans- 

 plants on artificial media are readily made, but some difficulty is often en- 

 countered in securing growth from experimental lesions. This difficulty is greatest 

 when large quantities of the material are transferred to the media, probably 

 because of an inhibiting material in the contents of the lesions. This hypothesis 

 is supported by the fact that development is much more easily secured if the 



