452 MUSGRAVE, CLEGG, AND POLK. 



granules from the lesions are washed in sterile salt solution before transferring 

 them to artificial media. Growth on satisfactory media becomes apparent in 

 from two to four da^s; development gradually continues for several weeks, 

 depending somewhat upon the quantity of medium present. The principal 

 characteristics of growth on various types of media are shown in the table. 



Pathogenicit y : Several monkeys have been successfully infected with the Foul- 

 erton and Strong strains of this organism, but results with the one from the 

 Pasteur Institute were all negative. Intra-abdominal inoculation has proved 

 the most satisfactory and under similar conditions the character of the lesions 

 produced varies but little. The lesions consist of nodular, tubercle-like processes 

 in the omentum, abdominal wall, and contiguous tissues. These lesions break 

 down and become surrounded by pockets and channels containing a peculiar, 

 pus-like material in which may be found the microorganisms. (See also animal 

 experiments. ) 



No. 3. Streptothrix madurce. (Pasteur Institute culture.) This is identical 

 in all essentials with 8. Vincent except that it is not pathogenic for monkeys. 

 This may be due in part at least to its long cultivation on artificial media. 



No. 4. Streptothrix madurw. (Strong's culture.) This organism is identical 

 with Vincent's species and its description has been included in the above. 



No. 5. Streptothrix a-ctinomyces. This culture was received from Professor 

 Binot of the Pasteur Institute as human Actinomyces. It is quite different from 

 the Wright type and it also appears to have slight distinctions from the Bostroem 

 type of Actinomyces. 



Morphologically it appears in the tissues as a fungus of the ray type, but 

 without clubs in young experimental lesions. In colonies the cultures are dark, 

 almost black in color, are made up of the branching filaments and transforma- 

 tion products, generally with radial arrangement of the terminals at the margins 

 of the colonies. 



The- organism stains poorly by the usual laboratory methods; it is Gram 

 positive and neither acid nor alcohol fast. 



Culturally it is a facultative aerobe and grows on the majority of the usual 

 media. In reclaiming cultures from experimental lesions this organism acts 

 very much like 8. madurae mentioned above; however, if the colonies are first 

 washed in distilled water and then transplanted to artificial media, growth 

 takes place without difficulty. 



This type, like the majority of the species examined, grows better in media 

 containing sugar or glycerine. Pigment is produced in all media and the body 

 of the medium is generally darkened to a considerable extent by the culture. 

 The pigment varies from an ochre-yellow in ordinary agar to a dark, almost black 

 color in glycerine-agar and on potato. 



The characteristics on varous media are shown in the table. 



Pathogenicity : This species is pathogenic for monkeys by intraperitoneal in- 

 oculation. It produces the usual actinomycotic lesion like those seen in the 

 clinical disease in man and animals. The organisms are found in the broken- 

 down mass discharged from the sinusses. 



No. 6. Streptothrix of farcin du ooeuf "A," and 



No. 7. Streptothrix nocardii. These two strains are identical in all essential 

 particulars and may therefore be described together under the correct name of 

 8. nocardii. 



Nocard's original description of this organism was not very complete, but 

 several other observers have studied his and other strains and a number of good 

 descriptions have been given. Foulerton has devoted careful study to this 

 6pecies and our work is in general confirmatory of his description. We have 



