Dtothrix caprse Silberschmidt. 



Our culture 



Short filaments resembling 

 coid forms ; occasionally Ion 

 ing branching but no club fc 



Streptothrix freeri. 



Cultures from 2 weeks to 2 m 

 Ziehl-Neelsen method show 

 tions,. giving the organism 

 short, plump bacilli. Gram 

 staining. 



Growth appears in 2 or 3 day 

 colonies ; medium remains n 

 so well nor does it produce 

 medium that it does on tl 

 media. 



Growth appears after 2 days 

 white colonies which later 

 growth a moist, mealy appef 

 brownish color. 



Similar to glycerine-agar.. 



Appear after 3 days as sligh 

 which soon assume a light-bro 



Colonies develop on the surfac 

 and later form a surface mi 

 forms at the bottom of the 

 mains clear. 



Very slight growth ; no pigme 



Pathogenic for guinea pigs an 

 cutaneous and intraperitone 



Our culture. 



Long, branching filaments, 2 to 7 fi in diameter. 

 Transverse segments are shown of various 

 length from coccoid to 10 y, in length. Branch- 

 ing occurs as lateral hypha developing from 

 the segments. The filaments have a definite 

 wall. Spores have not been observed. Coc- 

 coid and club-like forms present in certain 

 medium. 



Culture from 5 days to 2 months stained by 

 Ziehl-Neelsen method show many acid-fast 

 portions. The organism is Gram-positive. 



From 2 to 3 days' growth appears as smooth, 

 glistening colonies which later coalesce and 

 produce a delicate pink pigment ; medium 

 remains moist. 



Growth after 3 to 4 days at first as small, 

 whitish colonies ; these gradually take on a 

 delicate pink color ; later as the colonies 

 develop they become umbilicated and coalesce 

 forming a heaped-up growth and produce a 

 burnt-ocher color. 



Similar to glycerine-agar. 



Growth appears after 3 days as flat particles on 

 the surface of the medium ; these produce in 

 time a delicate pink color ; as growth proceeds 

 a granular mass collects in the bottom of the 

 tube ; this mass is more or less coherent. 



Very slight growth ; 



pigment ; no clubs. 



Pathogenic for guinea pigs and monkeys by in- 

 traperitoneal injections. 



