274 The Structure of Colored Blood-Corpuscles. 



amples, I will quote from my JSTote-book a few words referring 

 to the examination of the colored blood-corpuscles of the ox 

 and the newt — the one an example of the unnucleated, the 

 other of the nucleated corpuscles. 



A drop of fresh ox blood, mixed with a 50 per cent, satur- 

 ated solution of bichromate of potash, and highly magnified 

 (Tolles' T V immersion) exhibited, within 20 minutes, vacuoliz- 

 ation beginning in several red corpuscles. Within 40 min. 

 utes, knobs were protruded, though not copiously. In the 

 course of an hour, " paling " proceeded regularly, so that the 

 network became visible in some, and within two hours, in a 

 large number, of the corpuscles. After three hours, the net- 

 work, the Note-book says, was very distinct in many corpus- 

 cles, with some detritus and a few "ghosts." Twelve hours later, 

 about one-half of the whole number of corpuscles showed the 

 reticulum, while the other half were either vacuolized or un- 

 changed. No further change was observable for two days. 

 After the third day, some few corpuscles, perhaps, that had 

 not shown the network structure before, now did ; but the 

 paled ones had become too pale to do so, except a very few 

 which showed it finally. The rest had become " ghosts," 

 with much detritus. A week later, nearly all the corpuscles 

 that had exhibited the network had become " ghosts," only in 

 a very few of which, faint traces of the reticulum could be 

 made out. The rest were still unchanged, as on the first day 

 and remained so as long as the specimen was kept. 



The red blood-corpuscles of the newt, examined in a 50 per 

 cent, saturated solution of bichromate of potash, into which a 

 drop of the blood from the freshly cut tail had been allowed 

 to fall, presented peculiar changes of shape, consisting mainly 

 in contractions of the body around the nucleus. 



The nuclei always exhibited the network structure, either 

 perfect, and more distinct than in specimens unmixed with 

 the solution, or, when the nucleus was swelled to double or 

 treble its original size, with the network torn. Just as in the 

 case of the colorless corpuscles, there were seen two kinds of 



