The /Structure of Volored Mood- Corpuscles* £95 



asserts that the nucleus of nucleated bloocLcorpuscled does 

 not exist during life, but is a product of decomposition after 

 death. Likewise /Savory, in a paper 1 read before the London 

 Royal Society, urged that " when living, no distinction of 

 parts can be recognized ; and the existence of a nucleus in 

 the red corpuscles of ovipara is due to changes after death, 

 or removal from the vessels;" and furthermore, " the shadowy 

 substance seen in many of the smaller oviparous cells after 

 they have been mounted for some time, is very like that seen 

 under similar circumstances in some of the corpuscles of 

 mammalia." But Bottcher has reported 2 seeing nucleated 

 blood corpuscles in the capillaries of living frogs, and more 

 recently Hammond saw a nucleus in the red blood-corpuscles 

 of young trout, varying as to age from a day to three weeks^ 

 swimming in a cell full of water 3 ; and, afterward, also in those 

 of the tail of frog-embryos and in other animals 4 . 



Bottcher has by numerous methods and for a long time 

 sought to demonstrate the existence of a nucleus in mamma- 

 lian red blood-corpuscles. In his first publication 5 he gave a 

 historical sketch of the literature of the subject, and described 

 the effects of chloroform, magenta, tannin, and other reagents. 

 He also treated corpuscles with serum of other blood ; next 6 

 he placed them in aqueous humor (" methods which alter the 

 red blood-corpuscles as little and as slowly as possible ") ; 

 afterward 1 he treated them with alcohol and acetic acid, and. 



(1) " On the Structure of the Red Blood-corpuscle of Oviparous Vertebrate." Proceedings 

 of the Royal Society, XVII, 1868, 1869. (Read March 18, 1869.) Monthly Microscopical 

 Journal, April, 1869, p. 235. 



(2) "Untersuchungen liber die rothen Blutkorperchen der Wirbelthiere.*' VirchoVs Ar= 

 ohiT, vol. 36 (1866). (pp. 342-423), p. 351. 



(3) " Observations on the structure of the red blood-eorpuscles of a young trout." 

 Monthly Microscopical Journal, June, 1876. pp. 282-283. 



(4) "Observations on the structure of the red blood-corpuscle* of living pyrenasma- 

 toua vertebrates." Id., September, 1876, p. 147. 



(5) The "Untersuchungen " just cited, pp. 359, 363, 367, etc., and 376. 



(6) " Nachtragliche Mittheilung iiber die Entfarbung rother Blutkorperchen und iiber 

 den Nachweis von Kernen in deuselben." Virchow's Archiv, vol. 39 (1868), pp. 427- 

 435. 



(7; "Neue Untersuchungen iiber die rothen Blutkorperchen." Memoires de l'Acad. Imp. 

 des Sci. de St. Petersbourg, VII Ser., t. 22, No. 11. 



