14 F- J- F- Barringf.on, 



or had a thin layer of mucin adhering to the free ends of the cells. 

 The contrast with the control gland was best seen when this was rich 

 in mucin , as in late pregnancy or the early Puerperium. Exhaustion 

 appeared to occur with equal facility in either of these two cases though, 

 as previously shown, they are in different phases of activity. In such a 

 case the tall, columnar cell with a compressed, deeply stained nucleus 

 at the base, giving a marked reaction with mucin stains, became much 

 shorter or even cubical, with a clear, round nucleus having a well marked 

 nucleolus and chromatin network, and gave no reaction with mucin 

 stains. The cell protoplasm on the exhausted side stained rather more 

 deeply with iron haematoxylin, whereas on the normal side this hardly 

 stained at all: hence with this stain alone, the separation between ad- 

 jacent cells was more obvious on the normal side ; but when both where 

 stained with muci-carmine in addition, this stain more or less obscured 

 the boundaries on the normal side but left the exhausted cells unaf- 

 fected, so then the separation was more obvious on the stimnlated 

 side. When small quantities of mucin remained in the cells of the sti- 

 mulated side, it usually occured at their free ends near the lumen. 

 A gland exhausted in this way differed from one normally containing 

 minimal quantities of mucin, e. g. in lactation, in having larger cells, 

 all the acini contracted and none of the homogeneous kind of secretion 

 in any of the acini. These effects could not be produced in cats whose 

 inferior splanchnic nerves had been divided at their entrance into the 

 inferior mesenteric ganglion six to eleven days previously. In one ex- 

 periment the hypogastric was stimulated for five and a half hours 

 and nicotine injected intravenously at intervals, no exhaustion was 

 produced. This method however was not satisfactory, as after the first 

 fifteen minutes, it was found impossible to completely abolish the 

 cervical sympathetic effect on the pupil, by which the injections were 

 controlled, even with very large doses, and further, the enormous quan- 

 tity of nicotine necessary to keep this effect minimal — over 0,75 

 grams were given in the eight hours — produced a general condition 

 which it seemed probable, would militate against the free secretion 

 of a gland wherever the gangha of its nerves were situated. These 

 facts show that while both hypogastric and pelvic visceral nerves 3on- 



