330 E. T. Bell, 



5 days. Killed, July 25. There is a marked increase in the size and 

 staining intensity of all the liposomes. They are now readily visible in 

 unstained preparations, and a large number may be colored brown by 

 osmic acid. Fig. 8 shows a dark fiber from the plug taken July 20. 

 Fig. 9 shown a dark fiber of the opposite gastocnemius taken July 25. 

 Both preparations were stained with Herxheimer's solution. The marked 

 increase in size and staining-intensity of the liposomes will be apparent. 



Aug. 4. Leopard frog, caught in the open field. Plug shows a few 

 faint hposomes in the dark fibers, none in the light fibers. Fed heavily 

 on fat meat and olive oil. Killed Aug. 10. All the fibers are loaded with 

 liposomes. Those in the dark fibers are large and stain with considerable 

 intensity. 



Aug. 4. Young leopard frog, caught in open field. Weight, 12.5 g. 

 Plug shows no liposomes at all in the fibers. Fed heavily on fat meat. 

 Killed Aug. 10. All the fibers are loaded with liposomes similar to those 

 just described. 



Aug. 4. Young bull frog (Rana catesbiana) caught in the open 

 field. Weight 13.3 g. Plug shows no liposomes at all. Fed heavily on 

 olive oil and fat meat. Killed, Aug. 9. Fibers are all full of liposomes. 

 There are only a few dark fibers. The liposomes in these are larger and 

 stain more intensely than those in the pale fibers. Altmann's solution, 

 24 hours, stains most of the liposomes a very light brown. Some are 

 visible but not appreciably colored. 



Aug. 1. Leopard frog. Weight 35.5 g. Plug shows that the granules 

 are few and very faint. Fed heavily on grape sugar. Killed Aug. 10. 

 The liposomes are unchanged. 



July 21. Leopard frog. Weight 32 g. Plug, shows that the Hpo- 

 somes are all very faint. Fed starch paste. Killed July 27. The lipo- 

 somes are unchanged. 



Aug. 1. Leopard frog. Weight 32.1 g. Plug shows a large number 

 of very faint hposomes. Fed pure lean meat four days. The liposomes 

 are unchanged. 



Aug. 15. Bull frog. Weight, 41.3 g. Plug shows a considerable 

 number of faint liposomes. Fed heavily on palmitic acid^). Plugs of 



^) Palmitic acid and sodium oleate are solids, and it is difficult to feed them. 



