332 E. T. Bell, 



Aug. 18, 1910. Brown rat, weight 192 g. Fed a little grain each 

 day but gradually reduced to 139 g. by Aug. 26. Loss of weight, 

 27.6 per cent. Full fed on fat meat until Sept. 22, on which date it 

 was killed. The rat gained slowly until. Sept. 6, attaining a weight 

 of 159 g. It maintained an approximately constant weight from that 

 time. When killed it was still 18 per cent below its normal weight, 

 but microscopic examination shows all the fibers filled with liposomes. 

 Herxheimer's solution stains the droplets in the dark fibers a deep red ; 

 those in the light fibers, a pale red. Altmann's osmic-bichromate colors 

 many of the liposomes in the dark fibers a dark brown ; those in the pale 

 fibers are not stained. A great many more liposomes were shown in this 

 rat than are to be found in the other rats in the laboratory that were not 

 specially fed. 



Aug. 27. Brown rat. Weight 188 g. Reduced gradually to 149 g. 

 by Aug. 31. Loss of weight 20.7 per cent. Fed a mixed ration of bread, 

 milk, fat and lean meat, etc. Weight Sept. 6, 181 g. Microscopic exami- 

 nation shows all the fibers full of liposomes essentially similar to the 

 one just described. Some of the liposomes in the light fibers stain a 

 light brown in Altmann's solution. 



Aug. 27. Brown rat. Weight 191 g. Reduced gradually to 144 g 

 by Sept. 1. Loss of weight, 24.6 per cent. Fed a mixed ration. Weight 

 Sept. 7, 187 g. The fibers are loaded with liposomes similar to those of 

 the two preceding. 



Aug. 22. Brown rat. Weight 205 g. Reduced to 140 g. by Aug. 29. 

 Loss of weight, 31.2 per cent. Full fed on lean meat until Sept. 7, when 

 it was killed. Final weight 188 g. The fibers are all full of liposomes. 

 Those in the dark fibers stain a bright red ; those in the light fibers, a 

 pale red. Altmann's solution colors a few liposomes in some of the 

 dark fibers a light brown. 



Several rats were reduced in weight but could not be made to 

 increase, and died in this condition. These showed practically no lipo- 

 somes. Extreme hunger produces marked degenerations in the kidney, 

 liver and other organs ; and it is usually difficult to bring the animal back 

 to its normal weight. The less the reduction, the quicker the animal 

 recovers. 



