

184 G. G. Heslop: 



Antigen 2. — An alcoholic extract of three months' old culture 

 of the organism of contagious pleuro-pneumonia in Martin's 

 broth horse serum. On testing, this extract was found to possess 

 no demonstrable antigenic value. 



Antigen j. — An alcoholic extract of normal ox heart muscle. 

 On testing, this extract was found to possess no demonstrable 

 antigenic value. 



Antigen ^. — An alcoholic extract of guinea-pig's heart muscle. 

 On testing, this extract was found to possess only slight antigenic 

 value. No fixation of complement occurred with known negative 

 sera, while, with known positive sera, in three out of ten samples 

 tested there was slight inhibition of haemolysis. The remaining 

 seven positive samples gave negative reactions. 



Antigen 5. — An alcoholic extract of diseased lung taken from 

 an active case of contagious pleuro-pneumonia. On testing, this 

 extract was found to possess no demonstrable antigenic value. 



Antigen 6. — An alcoholic extract of fresh sub-epidermal 

 tumour tissue removed from behind the shoulder of calf 10 

 after an experimental subcutaneous inoculation of pure virus 

 in that region. On testing, this extract was found to possess 

 a fairly high antigenic value. No fixation of complement oc- 

 curred when it was tested with 10 different samples of known 

 negative sera, while with 10 different samples of known positive 

 sera, nine showed fixation of complement varying from partial 

 to complete fixation, the other known positive serum reacted 

 negatively. This extract was unsatisfactory in that it was highly 

 anti-complementary in any quantity tested of a 1 in 10 dilution 

 with saline. Even in a 1 in 20 dilution it was anticomplementary 

 excepting in the smallest amounts. 0.05 c.c. of 1 in 20 dilution 

 was the unit used for the tests already referred to. 



Antigen 7. — An alcoholic extract of the dried residue of eva- 

 porated culture (Antigen 1), to which was added 0.4 per cent, 

 chlosterin. 



Antigen 8. — An alcoholic extract of three months* old culture 

 in Martin's broth horse serum (Antigen 2) to which was added 

 0.4 per cent, chlosterin. 



Antigen p. — An alcoholic extract of ox heart muscle (Antigen 

 3) to which was added 0.4 per cent, chlosterin. 



Antigen 10. — An alcoholic extract of guinea-pig's heart muscle 

 (Antigen 4) to which was added 0.4 per cent, chlosterin. 



