1890,] occurring in the NeiglihourJiood of Simla. 91 



petioles were thus attacked, tho pustules breaking out from both sur- 

 faces of the leaf blade. At this time I found that the uredospores 

 germinated freely in water, throwing out a simple long tube (about 200/>t 

 in lengthy and 5/x in diameter), into which the coloured contents of the 

 spore wandered, leaving the walls of the latter colourless. In March 

 this stage is still common, but now the sori are more frequently erupted 

 from the lower surface of the blade, a few pustules occurring on the 

 upper surface, exactly opposite some below. Still only the lower leaves 

 near the ground are attacked. The sori on the lower surface tend to 

 coalesce now. Towards the end of March the uredospores do not germi- 

 nate so readily in water. The fungus is then missed to general observa- 

 tion until early in July, when a new crop of uredo pustules attracts 

 attention. These are numerously erupted from the upper leaf surface, 

 and now from the upper leaves on the stalks. At the same time such 

 attacked plants usually exhibit some generally paled lower leaves, on 

 the lower surfaces of which waxy orange red elevations may be seen, 

 which are teleutospore beds. The same leaves usually bear a few uredo 

 pustules as well. This stage continues throughout August ; but the 

 teleutospore beds increase in numbers whilst the uredo'pustules diminish 

 and become very scarce, though never entirely absent. At the end of 

 September a third crop of uredo pustules is produced, now all over the 

 green parts of the plant, ascending to, and involving even the green 

 parts of the flower and young fruit capsules. Shortly after this the 

 host withers and dries up. From July onwards the teleutospore beds 

 are constantly met with. 



The uredospores of all three crops are alike, both in measurement 

 and in general appearance (fig. 10, PI. IV). They are given off in 

 chains, are orange red, thick walled, beset with tubercles, and measure 

 on an average 21 x 17/*; but after lying in water for 12 to 24 hours 

 25 X 18/x. Each spore appears to have three germ pores. 



The teleutospores are covered by a well marked hyaline layer. The 

 spores are orange red and are divided by transverse septa into 3 to 5 

 cells (fig. 2, PL IV). The average length of each complete spore is 40 

 to 45/x, and the average breadth 14/x. 



'Remarks. — I have named this species provisionally G. Gampanulae ; 

 but it should be noted that both the uredo- and teleutospores are smaller 

 in the Simla species ; neither are the uredospores so variable in size and 

 shape a8 they appear to be in the European species. 



13 



