178 G. G. Heslop; 



used as the test culture. The result of the agglutination test with 

 this serum was as follows : — 



Serum No. 30. 



T . Culture Serum (diluted) Carbol Kesult at Result at 



xuoe - (undilJted). (1 in 1). Saline. 24 hours. 40 hours. 



1 



. 



c.c. 

 15 



- 



C.C. 



1.0 



c.c. 



10 



_ 



+ • 



. 



+ + 



2 



- 



,, 



- 



075 



125 



- 



+ 



- 



+ -f 



3 



- 



„ 



- 



5 



Lo 



- 



? 



- 



+ 



4 



- 



„ 



- 



025 



1-75 



- 



— 



- 



? 



5 



- 



,, 



- 



01 



19 



- 



— 





— 



6 



- 



» 



- 



006 



194 



- 



— 





— 



7 



• 



>» 



- 



004 



1*96 



- 



— 



- 



— 



8 



. 



,, 



- 



— 



2 



- 



— 



- 



— 



9 



- 



— 



- 



1 



2o 



- 



— 



. 



— 



10 



- 



1-5 

























Serum No. 17. 











1 



. 



c.c. 

 15 



. 



C.C. 



L 



c.c. 

 1 



. 



_ 









2 



- 



„ 



- 



075 



025 





— 



- 



— 



3 



• 



„ 



- 



05 



15 



- 



— 



- 



— 



4 



- 



,, 



- 



025 



1-75 



- 



- 



- 



— 



5 



- 



» 



- 



01 



19 



- 



— 



- 



— 



6 



- 



» 



- 



0-06 



1-94 



- 



— 



- 



— 



7 



- 



>> 



- 



004 



T96 



- 



— 



- 



— 



8 



- 



jj 



- 



— 



2 



- 



— 



- 





9 



- 



— 



- 



1 



25 



- 



— 



- 



— 



+ 4- = Agglutination and sedimentation. — = Negative reaction. 



+- = Recognisable agglutination. 1 = Doubtful reaction. 



In tubes 1 and 2 in the Serum-No. -30 series, agglutination was 

 perceptible after 24 hours' incubation at 37°C. The amount of 

 deposit in either tube was very small, but it could be clearly 

 recognised. Tube 3, after 24 hours' incubation, appeared to show 

 some agglutination, but it was so indefinite it could not be re- 

 corded as a " positive " result. When read after standing at room 

 temperature for a further period of 16 hours, tube 3 showed 

 agglutination, while tube 4 showed an indefinite agglutination 

 similar to tube 3 at 24 hours. Tubes 1 and 2, after standing at 

 room temperature showed agglutination and sedimentation, which 

 was very clearly recognisable. The reactions with Serum No. 17 

 were negative throughout, while all the controls were negative 

 also. 



Having obtained a positive agglutination reaction with the 

 serum of an animal which had been experimentally inoculated, the 



