182 G. G. Heslop: 



stopper. A length of india-rubber pressure tubing was attached 

 to the side arm of Flask A, the other end of the tubing being 

 attached to the side arm of Flask B. Flask B was empty, and was 

 closed at the mouth by an india-rubber stopper, which was per- 

 forated to give passage to a length of glass tubing, one end of 

 which extended to the bottom of Flask B. On the other end 

 of this glass tube another piece of india-rubber pressure tubing 

 was attached, and was connected to a piece of glass tubing 

 perforating the india-rubber stopper of Flask C, which was a 

 flask exactly similar to Flask B. A piece of india-rubber pres- 

 sure tubing connected Flask C by the side arm to the glass tub- 

 ing perforating the cork of a Sulphuric Acid Tower (D). The 

 sulphuric acid tower was connected by rubber tubing to an as- 

 pirator. (A Korting pattern water pump.) This aspirator main- 

 tained a negative pressure in Flasks A, B, C, and in the tower 

 D. Flask A, containing the culture was kept at room tempera- 

 ture. Flask B was placed in a receptacle containing crushed 

 ice and salt, the flask being quite covered by the freezing mix- 

 ture. Additional ice and salt were added from time to time as 

 required. The fluid, which collected in Flask B, was transferred 

 to Flask C by aspiration. At the end of 24 hours the apparatus- 

 was dismantled, and the contents of Flask A were measured T 

 and were found to be only 173 c.c.s. ; 251 c.c.s. of clear watery^ 

 solution having passed over into Flasks B and C. This 173 c.c.s.. 

 of concentrated culture showed a very marked turbidity, and 

 a quantity of it was used as an antigen (Antigen J) in the com- 

 plement fixation test, and a further quantity was used for ag- 

 glutination tests as follows : — 



First Test. 



Concentrated culture with Serum No. 26 (positive), and Serum 

 No. 7 (negative). 



Sebum No. 26 (Positive). 



T , Culture Serum (diluted Carbol Result at Result at 



(concentrated). 1 in 1). Saline. 24 hours. 40 hours. 



c.c c.c. c.c. 



1 - 15 



2 - „ 



3 - „ 



4 - „ 

 5 



6 - „ 



7 - „ ■ 



5 - „ 

 9 - — 



10 - 1 5 



1 



1 



075 



1-25 



05 



15 



025 



1-75 



01 



19 



006 



1 91 



0-04 



1-9(5 



— 



2 



1 



2.5 



