Pleuro-Pneumonia of Cattle. 



183 



Serum No. 7 (Negative). 



1 

 2 

 3 

 4 

 5 



7 



8 



9 



10 



o.o. 

 1 5 



1-5 



c.c. 

 1 



075 

 05 

 0-25 

 01 

 0-06 

 004 



1 



c.c. 

 1 



125 

 1-5 

 175 

 1-9 

 1-94 

 1-96 

 2 

 25 



— = Negative reaction. 



Second Test. 



Concentrated culture with Serum No. 23 (positive), and Serum 

 No. 31 (negative). 



This test was set up in exactly the same manner as the preced- 

 ing test (Test 1), and the result obtained was exactly similar to 

 that obtained with Test 1. 



Third Test. 



Concentrated culture with Serum No. 30 (from Experimental 

 Calf 1), and Serum No. 31 (negative). 



Serum No. 30 (Positive). 



Tube. 





Culture 



Serum (diluted 





Carbol 





Result at 





Result at 



(concentrated 



. 



1 in 1). 





Saline. 





24 hours. 





40 hours. 







c.c. 





c.c. 





c.c. 











1 



- 



1-5 



- 



1 



- 



1 



- 



+ + 



- 



4- + 



2 



- 



15 



- 



075 



- 



1-386 



- 



+ + 



- 



+ + 



3 



- 



,, 



- 



05 



- 



15 



- 



+ + 



- 



+ + 



4 



- 



M 



- 



25 



*- 



1-75 



- 



+ 



- 



+ 



5 



- 



tt 



- 



01 



- 



1-9 



- 



+ 



- 



+ 



6 



- 





- 



006 



- 



194 



- 



? 



- 



? 



7 

 8 

 9 



- 



»• 



- 



004 



- 



1-96 



2 



25 



- 



— 



- 



— 



." 







_ 



1 



. 



. 







_ 







10 



- 



1-5 

























s 



ERDM NO. 



31 



(Negative). 













c.c. 





C.C. 





C.C. 











1 



. 



1-5 



- 



1 



- 



1 



- 



— 



- 



— 



2 



- 



M 



. 



075 



- . 



1 25 



- 



— 



- 



— 



3 



- 





- 



Oo 



- 



15 



- 



— 



- 



— 



4 



. 



„ 



- 



0-25 



- 



1-75 



- 



— 



- 



— 



5 



. 





. 



01 



- 



19 



- 



— 



- 



— 



6 



- 





. 



006 



- 



191 



- 



— 



- 



— 



7 

 8 

 9 



- 



" 



- 



001 



- 



1.96 



2 



25 



- 



— 



- 



— 



. 







. 



1 



. 



. 



— 



_ 



— 



10 



- 



15 



















+ + 



_ 



Agglutination and sedimentatioi 



i. 



_ = 



= Negative 



reaction. 



+ 



= 



Agglutination. 









1 = 



: Doubtful Reaction. 



