Fungus of Lolium. 289 



the last wash lx)ttles, and thereby reaching the cylinders. During 

 the process of changing the bottles, the rubber connections with the 

 •cylinders were clamped, so that air could not reach them. 



H) Nutritire solutions. 



The control pot was watered with a nutritive solution, made up 

 according to the following formula : — 

 Ammonium nitrate, 0.5 gram. 

 Potassium di -hydrogen phosphate, 0.2 gram. 

 Calcium sulphate, 0.1 gram. 

 Magnesium sulphate, 0.1 gram. 

 Sodium chloride, 0.1 gram. 

 Ferric cldoride, 0.04 gram. 

 Nitrogen-free water, 1000 ccs. 

 The second pot was watered with a similar solution, excluding 

 the ammonium nitrate. 



The chemicals used were the purest that could \)e obtained. The 

 watering solutions when ready for use gave negative results, with 

 the nitrogen tests already described. 



Results. 



August 18th. — Experiment commenced. 



August 27th. — Grains were germinating freely in lx)th pots. 



September loth. — The seedlings in the control pot were taller 

 and were showing a lietter colour than those deprived 

 of nitrogen. 



September 16th. — First signs of yellowing at tips of leaf in 

 nitrogen-free seedlings. 



September 19th. — All the seedlings in the nitrogen-free cylin- 

 der showed their first leaf distinctly yellow at tip, and 

 the yellow colour was extending back along the edges of 

 the lamina. Ihe seedlings were all in the two leaf stage. 

 The second leaf was quite green. The seedlings in the 

 control cylinder looked very healthy. No signs of dis- 

 colouration were evident in them. 



September 30th. — The nitrogen-free seedlings were about one- 

 third the height of the control seedlings. The first leaf 

 was very much discoloured and withered. T\\v second 

 leaf was still green, showing no signs of yellowing. 

 The third leaf just visible. The control seedlings were 

 healthy, and of a good green colour ; they showed 5-6 

 leaves. 



