294 Ethel McLennan : 



(9) The^vum is infected before any divisions have taken place- 

 in it. 



(10) The hyphae aggregate at the proximal end of the develop- 



ing grain. They are here used by the endospermic cells 

 in the embryonic pocket for food-supply for the develop- 

 ' ing embryo. 



(11) The association of the fungus with Lolium temulentum 



and Lolium ijerenne, is probably a well-marked case of 

 symbiosis, comparable in many respects with that met 

 with in Calluno vulgaris. 



(12) It has been suggested that nitrogen fixation was the function 



of the fungus, but an experiment has been performed, 

 and the result obtained showed that the fungus of 

 Lolium perenne is unable to fix nitrogen in the total 

 absence of external supplies of combined nitrogen. 



The foregoing work was carried out in the Botanical Depart-^ 

 ment of the Melbourne University during the years 1917, 1918, and 

 1919. I have to thank Professor Seward, Cambridge; Mr. S. F. 

 Armstrong, Agricultural School, Cambridge; Dr. Steward, Western. 

 Australia; Mr. Burtt Davey, South Africa; Mr. Breakwell, Sydney; 

 Vilmorin-Andrieux and Cie, Paris; and the Royal Botanic Gardens, 

 Kew, for forwarding supplies of grain from different parts of the- 

 world; also Mr. O'Brien, Assistant in the Botanical Department, 

 Melbourne University, for the help he has afforded in assisting^ 

 with experiments and taking photographs. To Professor Ewart 1 

 am indebted fori the facilities provided for the work, and for much, 

 helpful criticism during its progress. 



BIBLIOGRAPHY. 



(1) Vogl, A. E. — ^Zeitschrift fiir Nahrungsmitteluntersuchung, 



Hygiene und AVaarenkunde, Vol. XII., Jan., 1898. 



(2) Guerin, P. — *' Sur la presence d'un Champignon dans- 



I'lvraie.^'— Journ. de Bot., Vol. XII., Aug. 1898. 



(3) Hanausek, T. F. — ''Vorlaufige Mitteilung liber den von A. 



Vogel in der Frucht von Lolium temulentum entdeckten' 

 Pilz."— Ber der Deut. Bot. Ges., Vol. XVI. 1898. 



(4) Nestler, A. — " Tiber einen in der Frucht von Lolium temulen- 



tum, L., entdeckten Pilz."— Ber. der Deut. Bot. Ges., Vol. 

 XVI. 1898. 



