296 Ethel McLennan : 



(21) Duggar, B. M., and Davis, A. R. — '* Studies in the Physiology 



of the Fungi — I. Nitrogen Fixation.'' — Annals of the Mis- 

 souri Botanical Gardens, Vol. III., 191G. 



(22) Schramm, J. R. — '' A Contribution to Our KnoAvledge of 



the Relation of Certain Species of Grass Green Algae to 

 Elementary Nitrogen." — Annals of the Missouri Botanical 

 Gardens, Vol. I., 1914. 

 (2:3) Koch, A.— ''Handb. tech. Myk.," by Lafar, Vol. III. 



(24) Goddard, H. N.^'' Can Fungi Living in Agricultural Soil 



Assimilate Free Nitrogen?" — Botanical Gazette, Vol. LVL, 

 1913. 



(25) Anderson, V. G. — " The Determination of Nitrites in Water." 



— Soc. of Chemical Ind. of Victoria, Vol. XIII., 1913. 



(26) Freeman, E. M. — '' The Affinities of the Fungus of Lolium^ 



temulentum, L." — Annales Mycologici, Vol. IV., 1906. 



(27) Freeman, E. M. — '' Symbiosis in the genus Lolium." — Minne- 



sota Botanical Studies, 1904. 



(28) Hartley, H., Campbell, N. H., and Poole, R. H.— '' The Pre- 



paration of Conductivity Water." — Journ. of Chem. Soc.,. 

 Trans., 1908. 



EXPLANATION OF PLATES. 



All figures have been drawn with the aid of the camera 

 lucida (Zeiss). 



Plate XVIII. 



Figures 1-5 stained with aniline gentian violet, folloAved by 

 Gram's iodine wash. They illustrate the distribution of the fungus 

 in the grain, the position of the hyphae is indicated by the yellow 

 line shown in, the figures. 



Fig. 1. — A semi-diagrammatic representation of a transverse sec- 

 tion through the distal part of a grain of Lolium 

 perenne . 



(a) aleurone layer; (b) cells of aleurone layer, which 

 stain differently from the rest, probably ferment-contain- 

 ing cells; (e) starchy endosperm; (h) hyphal layer;, (h^) 

 coloured portions of hyphae (the great bulk of the layer- 

 is not stained with the gentian violet) ; (t) . pericarp and 

 testa. 

 Fig. 2. — A transverse section of the proximal end of the same 

 grain. Letters as in Fig. 1, and (s) scutellum; (el) epithe- 

 lial layer of scutellum. 



