Fungus of Lolium. 299 



Plate XXII. 



Illustrates detail of ovary at Stage B. The sections were stained 

 with Congo red, followed by gentian violet and Gram's iodine 

 -wash. 



^ig- 1. -^Detail of nucellus, showing network of hyphae. 



(n) disorganising nuclei; (h) hyphae stained with 

 Congo red; (hi) hyphae stained with* gentian violet; 

 (r) remnants of protoplasm of nucellar cells, x 1100 diam. 



Fig. 2. — Mode of branching of hyphae in nucellus. 



(n) nucleus; (v) vacuole; (b) origin of branch, x 1100 

 diam. 



Fig. 3. — Young endosperm cells showing the hyphal threads enter- 

 ing and being absorbed by them. 



(n) nucleus of endosperm cell; (p) protoplasm of endo- 

 sperm cell; (h) hyphae; (hi) hyphae in process of absorp- 

 tion. X 1100 diam. 



Fig. 4. — Similar to Fig. 3, but section taken from another grain 

 and stained only with gentian violet. The protoplasm 

 remained unstained and the hyphae in the cells stained 

 brightly with the violet dye. 



(n) nucleus of endosperm cell; (h) hyphae entering cell; 

 (hi) hyphae in protoplasm of endosperm cell in process of 

 absorption. x 1100 diam. 



Fig. 5. — Same as 3 and 4, but shows the branches arising almost 

 at right angles to main hyphal thread, and entering the 

 endosperm cells. Lettering as before. x 1100 diam. 



Plate XXIII. 



A section of the endosperm taken from a grain at Stage C. It 

 was stained with congo red, gentian violet and Gram's iodine. The 

 ^outer edge of this tissue is included in the figure. The cells in this 

 area are young, and more or less cubical, being formed hy the div- 

 rsion of the outermost layer; starch has not yet appeared in them. 

 The older cells of the endosperm are large, and thin-walled, and 

 •starch is being deposited in the protoplasmic network. Hyphae are 

 being absorbed by the outer dividing layer, and serve as a source 

 'Of food-supply to the endosperm. 



(h) nucellar hyphae; (hi) hyphae being used by the endosperm as 

 food; (c) young dividing layer; (e) older endosperm cells; (s) starch 

 ;groups; (p) protoplasm. x 1100 diam. 



