38 David H. Dolley, 



Experiment M.-E. 33. The recipient. Weight 9,4 kg. Carotid- 

 jugular anastomosis. The recipient was bled 300 cc. from the femoral 

 artery and then transfused to the point of failing pulse of the 

 donor. The donor was then given 300 cc. of normal saline intra- 

 venously and the transfusion tube reopened to transfer all the thus 

 diluted blood of fatigue possible, the animals being about the same 

 size. Total time of operation thirty -five minutes. Killed after 

 four hours. The recipient was uncommonly drowsy and stupid after 

 the operation. 



Injections of lactic and paralactic acids and of mono-potassium 

 phosphate. — The experimental progress of the dosage will be apparent. 

 In the later amounts there can scarcely be any question in regard 

 to the sufficient quantity. Only the experiments are recorded in which 

 differential counts were made. The lactic acid used was of U. S. P. 

 strength. The sarcolactic acid was prepared by Merck. 



Experiment M.-E. 26. The puppy from the transfusion litter. 

 Weight 970 grams. It was given 1 cc. of a 1 °/ lactic acid solution 

 intravenously at one half hour intervals until seven doses were 

 administered. After each dose there was an increase in cardiac 

 and respiratory rate, followed by marked drowsiness which soon 

 wore off. Total dose, approximately 0,1 cc. per kg of body weight. 

 Killed two hours after the last dose. 



Experiment M.-E. 28. Puppy, three months old. Weight, 

 1,75 kg. Under chloroform anesthesia 70 cc. of a 2°/ lactic acid 

 solution were administered intravenously. Dosage approximately 

 1 cc. of the full strength acid per kg of body weight. Following 

 the drug the animal was drowsy, stupid, shivered and retched. 

 Killed after nineteen hours. The effect had entirely worn off. 



Experiment M.-E. 29. Puppy, two months old. Weight 2 kg. 

 Injected intravenously with 1 cc. of sarcolactic acid extracted by 

 myself from the muscles of a fatigued dog. This was diluted with 

 saline solution in the ratio of 1 : 50. There were no perceptible ill 

 effects. Killed after six and one half hours. 



Experiment M.-E. 30. Puppy from the same litter as Experi- 

 ment M.-E. 29. Weight 3,09 kg. Under ether anesthesia, intra- 



