278 'E. V. Cowdry, 



The distribution of mitochondria is dependent upon the shape of 

 the nucleus. When the nucleus is spherical they are generally distri- 

 buted evenly throughout the cytoplasm. When, however, it is notched 

 they are most frequently heaped up in the cytoplasm near the iden- 

 tation (fig. 4 b), the rest of the cytoplasm being free of mitochondria. 

 Occasionally they are distributed fairly uniformly in the cytoplasm 

 even though the nucleus be indented (fig. 4 a). 



The amount of mitochondria is more variable than in the 

 lymphocyte. Attempts to count them failed. One obtains the impres- 

 sion, nevertheless, that the ratio of mitochondria to cytoplasm is less 

 than it is in the lymphocyte (compare fig. 4 with fig. 3). 



No centrosome, azurophile granules or granules of segregation 

 could be seen. 



Here, again, the observations were controlled by the study of 

 smears prepared in a variety of ways. 



5. Finely granular leucocyte (fig. 5a and b). 



Synonyms: neutrophile leucocyte and polymorph. 



Mitochondria could not be distinguished, unstained, from the 

 specific granulations. 



The permeability to janus green is far less than that of any of 

 the other cells described above, with the possible exception of the 

 red blood corpuscle. Mitochondria may be stained with janus green. 

 The nucleus generally stains a diffuse pinkish blue color at the 

 same time. Contrast this with the behavior of the nucleus of the 

 lymphocyte. 



The mitochondria may also be stained with diethylsafranin. 



Morphologically, the mitochondria are slightly smaller than those 

 already described. They are in the form of granules and rods. Fila- 

 ments do not occur. They are slightly larger than the specific granu- 

 lations. Occasionally several very large mitochondria may be seen, 

 as represented in fig. 5 a to the right hand side of the lobated nucleus. 

 The mitochondria appear side by side with the neutrophilic granules 

 from which they may be easily distinguished. 



The distribution appears to be uniform, but this cannot be said 

 definitely because of the obscuring effect of the specific granulations. 



