280 E. V. Cowdry, 



No results were obtained with diethylsafranin. 



The mitochondria do not differ in their morphology from those 

 occurring in other cells. 



The distribution could not be clearly made out on account of the 

 presence of the coarse granulations. 



The amount is apparently subject to considerable fluctuation. In 

 the cell represented in fig. 6 a they are fairly abundant, in that shown 

 in fig. 6b they are practically absent, while in certain other cells 

 none could be detected. 



The fixed and stained smears do not yield reliable results since 

 both the mitochondria and the coarse granulations are acidophils 

 Consequently no tinctorial differentiation was possible by the methods 

 of technique employed, e. g. the Altmann method, Bensley's anilin 

 fuchsin methyl green method and iron hematoxilin. 



7. Mastleucocytes. 



Synonyms: Basophile leucocytes, degenerating leucocytes (Minot 

 '12, p. 515). 



The technical difficulties in the recognition and study of these 

 cells with janus green and diethylsafranin are at present too great to 

 justify any statements. 



The following deductions are based on the observations recor- 

 ded above: 



The negative results obtained in the study of red blood cells may 

 be interpretated in two ways: (1) that, owing to the relative imper- 

 meability of the cell to janus green, the mitochondria could not be 

 stained even if they were present; and (2) that the mitochondria are 

 absent. 



In support of the first interpretation we have the failure of janus 

 green to stain the cytoplasm of nonnucleated red blood cells. In 

 addition, the argument from analogy, that Duesberg's ('10, p. 615) 

 observation that hemoglobin prevents the staining of mitochondria in the 

 fixed red blood cells of chick embryos, may be advanced as indicating 

 the possibility that here also the hemoglobin prevents the staining. 



The evidence in favor of the absence of mitochondria is stron- 

 ger. It is: 



