Tili; relut ions oi' iiiitoclioiidi i:i and ofiior (•yt()|)l:i.siiiic cijii.stituciif.s etc. 48ij 



tlie iiiiniit(^ I'od-likc iiciUMtsdincs nie (iiiitc indisi iirjiiislialil»' iVtDii iiiitft- 

 ('lioiidria. 



2. Altmaiin and iron lieiuato.\.\ liii iiictliods, almost identical with 

 those used by Held for tlie study oi ncurosomes, are in every-day 

 use for the demonstration ut iiiitoc linmlria (Meves, 1908 and 1910a; 

 Sanissonow, 1910; et al.). 



3. The rods, or innu'osonies, fixed in Allinaiiirs thiid acrordiiiof to 

 Held may be stained by the folio-wing- mitoclioiidrial methods. 



a) The iron liematoxylin method of Heidenhain. 



b) Bensley's copper-ehrome-hematoxylin method, 



c) Bensley's acid fuchsin-methyl green metliod. 



4. Similarly, those fixed in potassium bichromate, as indicated by 

 Held, may also be stained by representative mitochondrial methods, viz. 



a) The iron hematoxylin method of Heidenhain, 



b) Bensley's acid fuchsin-methyl green method. 



c) The Benda method. 



That is, these type II neurosomes, after the fixations used by 

 Held, have exactly the same staining reactions as mitochondria. 



Finally, the relation of the neurosomes of Held to mitochondria 

 may be stated as folloAVS: the neurosomes are not a single separate 

 and distinct type of cell granulation ; for, in the first place, the minute 

 rods (type II) which he observed in his Altmann and iron hematoxylin 

 preparations are mitochondria; and in the second, the nature of those 

 seen in the erythrosin-m ethylene blue preparations (type I) is unknown. 



5. Nissl substance, canalicular apparatus, and neurofibrils. 



The mitochondria, the Nissl substance, the canalicular apparatus, 

 and the neurofibrils are structurall\" indei)endeut elenuMits occurring 

 in the cytoplasm of nerve cells. The first of them has already been 

 dealt with and the relations of the others will now be discussed. 



There is considerable confusion in the literature concerning the 

 relations of these components inter se. Goldschmidt believes that 

 there is a relation between the Nissl substance and the mitochondria; 

 for he states (1909, p. 107Ì that the mitochondria, chondriocontes. etc., 



