50 W. Krause, Zur Mikrotechnik. 



The quantity of acid should be sufficient to give a thickish liquid 

 when cold. 



This fixative is painted on to the cold slide with a brush, at the 

 time of using. The sections are then placed, and the slide left in thè 

 oven of a water-bath for some minutes (10 — 15 minutes I find suffi- 

 cient). The carbolic acid is thus evaporated, leaving a perfectly trans- 

 parent stratum of shellac on the slide. The sections are next freed 

 from paraffine in the ordinary way and mounted in balsam. 



This method is considered to be the best and simplest for fixing 

 stained sections. 



The shellac can be dissolved directly in carbolic acid, but then 

 the fluid must stand a long time in order to become clear, as it can- 

 not be filtered. For this reason it is preferable to dissolve first in 

 alcohol. 



