416 Wm. S. Marshall and C. Ï. Vorhies, 



there are noticeable a number of minute, darkly colored globules, most 

 abundant at the bases of the „secretory processes", projecting into 

 the lumen of the gland. These projections we have already found 

 present, exceptionally, in the twenty-four hour stage. In some of the 

 periods of activity later than this they were also seen, but were 

 sparsely scattered over the section with nothing like the regularity 

 with which they now occur. 



Conclusions. — Our study has, it appears to us, brought out the 

 following points which hold true for the spinning glands of the species 

 we studied, and would, we believe, in part or entirely, hold true for 

 the same organ in other Phryganeidae and probably in the Lepidoptera. 



1. The nuclei show an extreme case of branching, no branch, we 

 believe, anastomosing with another. 



2. Different types of nuclei might be selected from the cells in 

 the gland, but neither would these types be limited to any particular 

 portion of the gland, nor would they be distinct, since gradations could 

 easily be found connecting any one with the others. This does not 

 apply to the sizes of the cells, which are always smaller in the nar- 

 rower ends of the glandular portion, or to the shape of the nuclei in 

 the conducting portion of the gland, which are always quite distinct 

 from those in the glandular part, 



3. Each nucleus is continuous from any one extremity to all others, 

 and in no instance could we define any segmentation. 



4. The nuclein is not in long strands, Carnoy [i], in the nucleus, 

 but each piece or mass is separate from all the others. - 



5. There are no extended areas in the nuclei free from nucléoles, 

 Korschelt [10, 12], but these are fairly evenly distributed. 



6. Each nucleus contains many nucléoles which are of varying 

 sizes, and many small chromatin granules. Of this we have no proof 

 except what we get from the differentiation of the stains we used. 

 Flemming's triple stain gives to the larger bodies a decided safranin 

 color and stains the smaller granules violet. Acid fuchsin and methylen 

 green gives to the larger bodies the fuchsin color and the granules 

 stain a dark green (Meves [16]). 



1. The nucléoles may contain vacuoles (Mewes [16]). 



