46 

 SDH from Chlorella extract were unaffected when incubated 

 with SP-I N. silvestris specific antibody. Activity was not 

 found when QDH was assayed in N. silvestris and C. 

 sorokiniana crude extracts. 



Table 3-1. Specific activities from N. silvestris and C. 

 sorokiniana aromatic pathway enzymes. 



Specific Activity (nmol min"-"- mg"-"^ protein) 



Enzyme N. silvestris C. sorokiniana 



QDT 12.00 6.20 



SDH 60.00 6.00 



PAT 16.00 3.75 



ADH/NADP 1.00 NF 



ADH/NAD NF NF 



PDH/NADP NF NF 



PDH/NAD NF NF 



ADT 0.6 NF 



PDT NF NF 



NF, not found; Crude extracts were used for assay. 



Separation of activities 



Column chromatography was used to separate the enzymes 

 of interest for this study from N. silvestris and C. 

 sorokiniana. DQT and SDH of the common pathway were not 

 separable in N. silvestris or in C. sorokiniana after two 

 steps of column chromatography (DE-52 and HA) . Figure 3-1 

 shows a DEAE-cellulose chromatographic profile of 

 N. silvestris suspension cell extract with overlapping 



