ABBREVIATIONS 



ADH arogenate dehydrogenase 



ADT arogenate dehydratase 



AGN L- arogenate 



aKG a-ketoglutarate 



AMP ampicillin 



ANS anthranilate synthase 



ANS-1 aneuploid N. silvestris suspension cell line 



jSME beta mercaptoethanol 



BSA bovine serum albumin 



CHA chorismate 



CM chorismate mutase 



CM-52 carboxymethyl cellulose-52 cation exchange column 



CM- I chloroplast chorismate mutase 



CM- II cytosolic chorismate mutase 



DAHP 3-deoxy-D-araJblno-heptulosonate 7-phosphate 



DAHPS DAHP synthase, enzyme 1 of aromatic pathway 



DE52 (DEAE) diethylaminoethyl cellulose-52 anion 



exchange column 



DHQ dehydroquinate 



DHS dehydroshikimate 



DQT dehydroquinate dehydratase or dehydroquinase 



Ds-Co cytosolic DAHP synthase of plants 



Ds-Mn chloroplast DAHP synthase of plants 



DTT dithiothreitol 



E-cells cells of about two generations of exponential 



growth 



EDTA ethylenediaminetetraacetate 



EE-cells cells in continuous exponential growth 



EPPS (N- [2-Hydroxyethyl]piperazine-N' - [3- 



propanesulfonic acid] ;HPPS) 



EPSP 5 - enoipyruvyl shikimat e - 3 -phosphate 



E4P erythrose 4 -phosphate 



HA hydroxylapetite column 



HPLC high performance liquid chromatography 



HPP 4-hydroxyphenylpyruvate 



ICBR Interdisciplinary Center of Biotechnology Research 



IPTG isopropylthio-jS-D-galactoside 



KPO4 potassium phosphate buffer 



LB Luria-Bertaini medium 



LB-AMP LB plus ampicillin 



LES lag, exponential and stationary phases of growth 



MTT 3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetra- 



zolium-bromide; (thiazolyl blue) 



NADP nicotinamide adenine dinucleotide 



NTPS nucleotide triphosphates 



ix 



