5-3 Gel filtration chromatography of S-proteins 83 



5-4 Optimal PAGE activity staining for SDH in crude 



extract 86 



5-5 PAGE activity staining of purified SI- and S2- 



proteins 88 



5-6 S-proteins pH optima of optima for activity 90 



5-7 Temperature optima for S-proteins 93 



5-8 Thermal inactivation of S-proteins 95 



5-9 Substrate protection against thermal 



inactivation of S-proteins 97 



5-10 Saturation curves and double reciprocal plots 



for SP-1 100 



5-11 Saturation curves and double reciprocal plots 



for SP-II 102 



6-1 Precipitation of S-proteins with SP-I specific 



antibody on Ouchterlony plates 110 



6-2 Effect of SP-I specific antibody on SP-I and 



SP-II activities 113 



6-3 Comparison of Western blot and activity stained 



gels 115 



7-1 Agarose gels of cDNA encoding aromatic pathway 



proteins 121 



7-2 Preliminary alignment of translated sequences of 



clone SP3 with established DQT sequences 124 



7-3 Sequencing strategy for cDNA encoding the 



bifunctional protein 126 



7-4 Agarose gels of SP3 cDNA fragments during 



stages of subcloning 128 



7-5 Nucleotide and deduced amino acid sequence of 



cDNA coding for the bifunctional S-protein 131 



7-6 Multiple amino acid alignment of the AroD 



domain of AroD«E with its homologues 135 



7-7 Dendrogram of dehydroquinase homologues 137 



VI 



