LIST OF FIGURES 



Figure Page 



1-1 Aromatic amino acid biosynthetic pathway 3 



1-2 Multiple compartment at ion of pathways in plant 



cells 7 



1-3 Quinate catabolism and aromatic amino acid 



biosynthesis 11 



3-1 DEAE-cellulose chromatography of aromatic pathway 



enzymes from N. silvestris 48 



3-2 DEAE- Cellulose chromatography of aromatic pathway 



enzymes from C. sorokiniana 51 



3-3 Temperature optimum for activity of prephenate 



aminotransferase in Chlorella sorokiniana 52 



3-4 Growth curve of N. silvestris in suspension 



cultures 56 



3-5 Arogenate dehydratase activities followed 



throughout a growth curve of N. silvestris 58 



4-1 Elution profiles of SP-I and SP-II from Celite 



545 column chromatography 64 



4-2 Elution profiles of SP-I and SP-II from DEAE- 

 cellulose chromatography 67 



4-3 Elution profiles of S-proteins from an NADP* 



specific affinity column 70 



4-4 Celite 545 column chromatography overlap of 



arogenate dehydrogenase and SP-II 72 



4-5 Hydroxyl apatite column chromatography separation 



of arogenate dehydrogenase and SP-II 73 



5-1 Elution profile of SDH activities from a Celite 



545 column run with a shallow gradient 78 



5-2 Molecular weight determinations by SDS PAGE and 



by silver stain gels of the two S-proteins 81 



V 



