TABLE OF CONTENTS 



Page 



ACKNOWLEDGEMENTS ii 



TABLE OF CONTENTS iii 



LIST OF FIGURES V 



LIST OF TABLES viii 



ABBREVIATIONS ix 



ABSTRACT xi 



CHAPTERS 



I LITERATURE REVIEW AND RATIONALE 1 



Aromatic Amino Acid Biosynthesis in Plants.. 1 



Rational for Focus upon DQT and SDH 8 



Shikimate biosynthesis and quinate 



catabolism 9 



Multiple Species of Shikimate Dehydrogenase. 12 



The Post-Prephenate Pathway in Plants 16 



II MATERIALS AND METHODS 19 



Organisms and Extract Preparation 19 



Enzyme Assays 23 



Separation of Pathway Enzymes by 



Chromatography 27 



Purification of Pathway Proteins 29 



Polyacrylamide Gel Electrophoresis 33 



Kinetic Values 34 



Inhibition studies 35 



Antibodies 3 5 



cDNA cloning and sequencing 3 7 



Biochemicals 43 



III PHYSIOLOGICAL PROFILE OF AROMATIC PATHWAY 



ENZYMES IN PLANTS 45 



Results 45 



Discussion 59 



iii 



