Fig. 4-2. Elution profiles of SP-I and SP-II from 

 DEAE- cellulose chromatography. The third step of 

 purification of the two S-proteins was a DEAE-cellulose 

 chromatographic column equilibrated in EPPS buffer (see 

 Materials and Methods for details) . SDH (•) activity 

 remained coincident with DQT (O) activity for both SP-II 

 (panel A) and SP-I (panel B) . The proteins began to elute 

 at about 0.25MKC1 of the gradient (solid line) . Protein 

 profiles were followed at Ajgo nm (— ) • 



