71 



Table 4-1. 



Purification of SI protein from N. 

 silvestris suspension cultured cells, 











SDH 







DQT 









Vol 



Total 

 Protein 





















Purity 



Yield 





Purity- 



Yield 



Ratio 



step 



ml 



mg 



SA 



fold 



% 



SA 



fold 



% 



S/D 



Crude 



540 



605 



56 



1 



100 



12 



1 



100 



4.7 



Celite 



643 



206 



126 



2 



70 



25 



2 



65 



5.0 



CM- 52 



720 



49 



412 



7 



55 



94 



8 



59 



4.4 



DEAE-1 



279 



35 



561 



10 



53 



113 



9 



50 



5.0 



HA 



618 



8.7 



1,515 



27 



36 



257 



22 



28 



5.9 



DEAE-2 



106 



1.95 



4,609 



82 



24 



941 



78 



23 



4.9 



Aff-1 



88 



0.42 



14,500 



259 



17 



2,342 



195 



13 



6.2 



DEAE-3 



83 



0.16 



33,878 



605 



15 



7,365 



614 



15 



4.6 



Aff-2 



54 



0.07 



65,877 



1176 



9 



12,923 



1077 



12 



5.1 



S/D is shikimate dehydrogenase/dehydroquinase 



Table 4-2. Purification of S2 protein from N. 



silvestris suspension cultured cells. 











SDH 







DQT 









Vol 



Total 

 Protein 





















Purity 



Yield 





Purity 



Yield 



Ratio 



step 



ml 



mg 



SA 



fold 



% 



SA 



fold 



% 



S/D 



Crude 



540 



605 



5 



1 



100 



1 



1 



100 



4.6 



Celite 



348 



97 



26 



5 



68 



5 



5 



64 



5.0 



CM-52 



345 



69 



31 



6 



59 



8 



8 



73 



4.4 



DEAE-1 



77 



31 



66 



13 



55 



14 



14 



53 



5.1 



HA 



104 



5 



295 



59 



43 



75 



75 



53 



5.4 



DEAE-2 



31 



1 



757 



151 



34 



187 



187 



34 



4.1 



Aff-1 



15 



0.006 



4,590 



918 



7 



880 



880 



7 



5.2 



S/D is shikimate dehydrogenase/dehydroquinate 



