72 



100 



\- 1-100 



(0 



T 60 



E 



I!t4 



CEUTE 545 



2.0 



1.0 



s 



CM 



280 300 320 340 



FRACTION NUMBER 



360 



Fig. 4-4. Celite 545 column chromatography overlap of 

 arogenace dehydrogenase and SP-II. Both SP-II (•) and ADH 

 (O) elute in the 45 to 0% ammonium sulfate gradient (solid 

 line) with overlapping activity peaks. Protein was 



monitored at A- 



290 nm 



(...) 



