73 



140 



120 



100 



JE 80 



E 



fc> 60 



40 

 20 







SDH 







"~ 



/ 





-v^^ 



Hydroxylapatite 



- 



r 





• N. 





— 







\ 



ADH /'"' - 



- 







\ 





1 







1 



/ \ " 





— 1™ 



-^ f— 



1 1 



:^ , ^V" 



600 

 400 



300 g 

 200 ^ 



100 

 



20 . 40 60 80 



FRACTION NUMBER 



100 



Fig. 4-5. Hydroxylapatite column chromatography 

 separation of arogenate dehydrogenase and SP-II. The SDH 

 activity (■) was found in the wash fractions and ADH (•) 

 eluted at about 120 mW KPO« . 



