Fig. 5-3. Gel filtration chromatography of S- 

 proteins. A) Concentrated crude extract (■) , pool IV 

 (SP-I) (•) and pool VI (SP-II) (a) (from the shallow 

 gradient Celite 545 column) were passed through a 

 Sephadex G-200 column. The dotted profile represents the 

 three marker enzymes (alcohol dehydrogenase, BSA and 

 carbonic anhydrase) . B) A molecular weight curve based 

 on the column profile from A suggests the molecular 

 weights for the SP-I to be about 59,000 and about 62,000 

 for SP-II. Crude extract S-protein is suggested to be 

 about 63,000. 



